Purification and characterisation of isocitrate dehydrogenase and malate dehydrogenase from Mycobacterium tuberculosis and evaluation of their potential as suitable antigens for the serodiagnosis of tuberculosis

Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
R Ohman, M Ridell

Abstract

Enzymes from Mycobacterium tuberculosis are potent antigens and might thus be of interest in the serodiagnosis of tuberculosis. The purpose of the study was to purify and characterize the two enzymes isocitrate dehydrogenase (IDH) and malate dehydrogenase (MDH) from, M. tuberculosis and to evaluate their potential in the serodiagnosis of tuberculosis. The two enzymes were analysed for specificity by electrophoresis and then purified by means of affinity chromatography using reactive dyes and ion exchange chromatography. The two isolated enzyme fractions were analysed by ELISA, using antisera against related organisms. They were then tested as antigens in ELISA together with sera from tuberculous patients and controls. The electrophoretical analyses showed that the two enzymes each differed markedly from the corresponding enzymes of other mycobacteria. The serological analyses, however, could not distinguish between either IDH or MDH from other mycobacteria, but organisms of other genera, such as Nocardia, gave much weaker responses. When IDH and MDH were tested with sera from tuberculous patients and controls the former gave clearly higher optical density values than the latter. The enzymes/antigens IDH and MDH may be of value ...Continue Reading

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Citations

Jun 17, 2009·PloS One·Tom A MendumJohnjoe McFadden
Jul 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jing TianCarl Nathan
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sharmistha BanerjeeSeyed E Hasnain
Sep 21, 2011·Acta Crystallographica. Section D, Biological Crystallography·Navdeep S SidhuGeorge M Sheldrick
Sep 16, 2018·Journal of Integrative Bioinformatics·Amit KatiyarKrishna Kant Azad
Nov 22, 1997·FEMS Microbiology Letters·M S HughesS D Neill

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