PMID: 8580769Jan 1, 1996Paper

Purification and characterisation of soluble invertases from leaves of Arabidopsis thaliana

Planta
X TangS A Rolfe

Abstract

Multiple isoforms of beta-fructofuranosidase (invertase, EC 3.2.1.26) were identified in mature green leaves of the cruciferous plant Arabidopsis thaliana (L.) Heynh. There were four major and one minor isoforms of soluble acid invertase and an additional activity which could be released from the cell wall by buffers of high ionic strength. This study reports the separation and characterisation of three soluble isoforms following ammonium sulphate and polyethylene glycol 6000 precipitations, Concanavalin A, MonoQ ion exchange, Superose 12 size-exclusion chromatography and chromatofocusing. These isoforms, designated INV1, INV2 and INV3, had isoelectric points of 4.75, 4.70 and 4.65 and a Km for sucrose of 5, 12 and 5 mM, respectively. Each had a pH optimum of 5.5, exhibited optimal activity at 45 degrees C and used sucrose as the preferred substrate. All fractions containing these isoforms contained a 52-kDa polypeptide which was specifically detected by immunoblotting with an antibody raised against deglycosylated wheat invertase. The N-terminal amino-acid sequence of this polypeptide was homologous to acid invertases isolated from other plant species. The possible origin of isoforms of soluble acid invertase is discussed.

Citations

Apr 21, 2005·Journal of Chemical Ecology·Steven D Allison, Jack C Schultz
Nov 11, 2009·The Journal of Biological Chemistry·Kohji YamadaKazuko Yamaguchi-Shinozaki
Jul 2, 2010·Journal of Integrative Plant Biology·Donggiun KimTaek-Kyun Lee
May 15, 2012·Plant Signaling & Behavior·Christoph Plieth
May 15, 2012·Plant Signaling & Behavior·Christoph Plieth, Sonja Vollbehr
Nov 20, 2014·Frontiers in Plant Science·Sophie TrouvelotMarielle Adrian
Aug 30, 2008·Science in China. Series C, Life Sciences·S LiangL J Feldman
Dec 13, 2005·Journal of Experimental Botany·Richard S QuilliamStephen A Rolfe
May 20, 2016·Journal of Experimental Botany·Carla Coluccio LeskowFernando Carrari
Feb 1, 2019·NPJ Systems Biology and Applications·Lisa KüstnerArnd G Heyer
Feb 20, 2003·Journal of Agricultural and Food Chemistry·Wen-Chin HuangHsien-Yi Sung
Apr 13, 2006·Journal of Agricultural and Food Chemistry·Chang-Wen HsiehAi-Yu Wang

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