Purification and characterisation of the BIOH protein from the biotin biosynthetic pathway

FEBS Letters
Nicholas H TomczykDominic J Campopiano

Abstract

Conversion of pimeloyl-coenzyme A (CoA) to biotin in Escherichia coli requires at least four enzymes encoded by genes in the bio operon. One gene, bioH, which is not present in the bioABFCD operon, is required for the synthesis of pimeloyl-CoA but its exact role in formation of this intermediate is unknown. To investigate this further, we have overexpressed and purified the bioH gene products from both E. coli (BIOH EC) and Neisseria meningitis (BIOH NM) in E. coli. When purified BIOH was incubated with excess CoA and analysed by electrospray mass spectrometry a species of mass corresponding to a BIOH:CoA complex was observed. Mutation of a conserved serine residue to alanine (BIOH EC S82A) did not prevent CoA binding. This is the first report of the purification of BIOH and the observation of a small molecule bound to the protein provides clues to its role in pimeloyl-CoA synthesis.

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Citations

Jan 16, 2003·Gene·Hiroyuki AkatsukaKenji Omori
Aug 10, 2010·Nature Chemical Biology·Steven LinJohn E Cronan
Sep 28, 2007·Natural Product Reports·Michael E WebbAlison G Smith
Jun 14, 2012·Protein Engineering, Design & Selection : PEDS·Humberto FloresEnrique Morett
Jul 13, 2007·Metabolic Engineering·Xinkai XieYi Tang
Dec 3, 2005·Protein Science : a Publication of the Protein Society·T Andrew BinkowskiJie Liang
Oct 12, 2004·Proteins·Sergei Izrailev, Michael A Farnum
Oct 13, 2018·Protein Science : a Publication of the Protein Society·Polytimi S DimitriouKonstantin Denessiouk
Mar 26, 2011·Molecular BioSystems·Steven Lin, John E Cronan

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