PMID: 2508758Oct 13, 1989Paper

Purification and characterisation of tyrosine decarboxylase and aromatic-L-amino-acid decarboxylase

Biochimica Et Biophysica Acta
T BørresenH Sørensen

Abstract

Microbial tyrosine decarboxylase (EC 4.1.1.25) and mammalian aromatic-L-amino-acid decarboxylase (EC 4.1.1.28) catalyse the formation of tyramine from L-tyrosine. These enzymes were characterised after isolation to purity by methods including fast polymer liquid chromatography (FPLC). Tyrosine decarboxylase was isolated from Streptococcus faecalis by FPLC anion exchange chromatography (11-times purification; 72% recovery; 23.2 U/mg protein). FPLC on Phenyl-Superose resulted in purification to 115 U/mg protein. Aromatic-L-amino-acid decarboxylase was isolated from pig kidney by ammonium sulfate fractionation, DEAE chromatography, and FPLC anion exchange chromatography (21-times purification; 22% recovery; 0.71 U/mg protein). By FPLC chromatofocusing, tyrosine decarboxylase eluted at pH 4.3 and aromatic-L-amino-acid decarboxylase at pH 5.0. Isoelectric focusing of tyrosine decarboxylase gave two bands (pI 4.4 and 4.5). With pyridoxal 5'-phosphate removed by ultrafiltration, only one band (pI 4.4) appeared, and SDS polyacrylamide electrophoresis confirmed the purity. FPLC gel filtration resulted in molecular weights 143,000 and 86,000, respectively, for tyrosine decarboxylase and aromatic-L-amino-acid decarboxylase. In SDS electro...Continue Reading

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Citations

Nov 12, 2013·Protein Expression and Purification·Kai Zhang, Ye Ni
Jun 8, 2002·FEMS Microbiology Letters·Patrick Lucas, Aline Lonvaud-Funel
Nov 5, 1999·FEMS Microbiology Letters·V Moreno-Arribas, A Lonvaud-Funel
Mar 1, 2012·Critical Reviews in Food Science and Nutrition·Angela MarcobalRosario Muñoz
Jul 29, 2011·Critical Reviews in Food Science and Nutrition·Daniel M LinaresMaría Fernández
Jun 29, 2002·Applied and Environmental Microbiology·Nathalie ConnilHervé Prévost
Dec 4, 2015·Chembiochem : a European Journal of Chemical Biology·Daniel KalbDirk Hoffmeister
Feb 20, 2007·International Journal of Food Microbiology·José María LandeteSergi Ferrer
Aug 9, 2006·Critical Reviews in Microbiology·María Fernández, Manuel Zúñiga
Dec 15, 2019·Cellular and Molecular Neurobiology·Katlynn Bugda GwiltGregory M Miller

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