Purification and characterization of a Bacillus megaterium disulfide reductase specific for disulfides containing pantethine 4',4"-diphosphate.

Journal of Bacteriology
R D Swerdlow, P Setlow

Abstract

An NADH-linked disulfide reductase specific for disulfides containing pantethine 4',4"-diphosphate moieties was purified 23,000-fold to homogeneity from spores of Bacillus megaterium. The enzyme had a native molecular weight of 122,000 with two apparently identical subunits, contained one molecule of flavin adenine dinucleotide per subunit, and was inhibited by the vicinal dithiol reagent arsenite. The enzyme was active only on disulfides containing pantethine 4',4"-diphosphate moieties, including pantethine 4',4"-diphosphate, oxidized coenzyme A, and coenzyme A in disulfide linkage to acyl carrier protein. However, the Km values for pantethine 4',4"-diphosphate and oxidized coenzyme A were 0.65 and 7.4 mM, respectively. The enzyme was at a low level in log-phase cells but increased up to 10-fold early in the stationary phase and had a similar specific activity in both the mother cell and the forespore compartment; the enzyme activity fell only slowly during spore germination and outgrowth. The enzyme was not detected in several eucaryotic sources and was present in at most a low level in a number of gram-negative bacteria. Surprisingly, the specific activity of this enzyme varied more than 200-fold in extracts from different B...Continue Reading

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Citations

Jul 22, 2008·Journal of Bacteriology·Carleitta PaigeAl Claiborne
Sep 7, 2001·Annual Review of Microbiology·R C Fahey
Jan 24, 2016·Applied Microbiology and Biotechnology·Sweety A WadhwaniBalu A Chopade
Nov 1, 1989·Journal of Molecular Evolution·A R Sundquist, R C Fahey
Oct 1, 1989·Bioscience Reports·R N Ondarza
Dec 20, 1996·Molecular and Biochemical Parasitology·D M BrownP Upcroft
Dec 22, 1998·Biochimica Et Biophysica Acta·H YounS O Kang
Jan 1, 1984·Journal of Bacteriology·E L Thomas
May 1, 1991·Journal of Bacteriology·J A PalmerJ R Sokatch
Apr 1, 2007·EcoSal Plus·Roberta Leonardi, Suzanne Jackowski

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