Purification and characterization of a Ca2+ -dependent prothrombin activator, multactivase, from the venom of Echis multisquamatus

Journal of Biochemistry
D Yamada, T Morita

Abstract

We previously found a novel Ca2+-dependent prothrombin activator, designated as carinactivase-1, in Echis carinatus leucogaster venom [D. Yamada, F. Sekiya, and T. Morita (1996) J. Biol. Chem. 271, 5200-5207]. Of the Viperidae snake venoms examined, the Echis multisquamatus venom had the strongest carinactivase-like activity. We isolated and characterized the carinactivase-like prothrombin activator in E. multisquamatus venom. From 50 mg of E. multisquamatus venom, we isolated 2.3 mg of a Ca2+-dependent prothrombin activator designated as multactivase. Unlike other Echis snake venoms, the E. multisquamatus venom contained no ecarin-like Ca2+-independent prothrombin activator. The structure and function of multactivase are similar to those of carinactivase. Multactivase is composed of a catalytic subunit with metalloprotease activity and a regulatory subunit comprising two homologous polypeptides bound by S-S bridge(s), and it activates prothrombin via recognition of the Ca2+-bound conformation of its Gla domain. We developed a chromogenic assay involving multactivase for normal prothrombin activity in plasma from individuals orally administered anticoagulants. The normal prothrombin activity, as a percentage, measured with mult...Continue Reading

Citations

Dec 18, 2002·Archives of Biochemistry and Biophysics·Rong GaoP Gopalakrishnakone
Nov 2, 2001·Toxicon : Official Journal of the International Society on Toxinology·Jeremiah S JosephR Manjunatha Kini
Nov 17, 2001·Toxicon : Official Journal of the International Society on Toxinology·Han-Seung JooChung-Soon Chang
Dec 16, 1998·Thrombosis Research·J W Govers-RiemslagG Tans
Jun 6, 2009·Cellular and Molecular Life Sciences : CMLS·R Doley, R M Kini
Sep 25, 2012·Toxicon : Official Journal of the International Society on Toxinology·Francis S Markland, Stephen Swenson
Jan 26, 2007·Toxicon : Official Journal of the International Society on Toxinology·Adrijana LeonardiIgor Krizaj
Aug 11, 2004·Toxicon : Official Journal of the International Society on Toxinology·Run-Qiang ChenYu-Liang Xiong
Jan 26, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·O H P Ramos, H S Selistre-de-Araujo
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Takashi Morita
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·R Manjunatha Kini
Mar 31, 2015·Toxicon : Official Journal of the International Society on Toxinology·Rupamoni ThakurAshis K Mukherjee
Jul 13, 2006·The Biochemical Journal·R Manjunatha Kini

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