PMID: 9175557Jul 1, 1997Paper

Purification and characterization of a cysteine protease produced by pathogenic luminous Vibrio harveyi

Current Microbiology
P C LiuS N Chen

Abstract

The purification and characterization of an extracellular protease produced by pathogenic luminous Vibrio harveyi strain 820514, originally isolated from diseased tiger prawn (Penaeus monodon), was presented in this paper. The purification steps included ammonium sulfate precipitation, with columns of hydrophobic interaction chromatography and anion exchange on fast protein liquid chromatography. The protease is an alkaline cysteine protease, heat labile, inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, p-chloromercuribenzoate, and p-chloromercuribenzene-sulfonic acid, and showed maximal activities at pH 8 and 50 degrees C, having a molecular mass of 38 kDa as estimated by SDS-PAGE and gel filtration column. In addition, the protease was also completely inhibited by CuCl2 and HgCl2, but not or only partially inhibited by other inhibitors tested. Furthermore, 2-mercaptoethanol was the most effective reducing agent in the activation of the enzyme. The present protease is the first cysteine protease found in Vibrio species.

Citations

Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·Nancy Busico-Salcedo, Leigh Owens
Feb 27, 2001·Insect Biochemistry and Molecular Biology·G ColebatchP Cooper
Jun 27, 2001·Applied and Environmental Microbiology·X H ZhangB Austin
Feb 8, 2006·Applied and Environmental Microbiology·Krit KhemayanTimothy W Flegel
Jun 4, 2004·Journal of Bacteriology·Jennifer M Henke, Bonnie L Bassler
Oct 20, 2012·PloS One·Vorrapon ChaikeeratisakAnchalee Tassanakajon
Dec 7, 2010·Journal of Microbiological Methods·Sathish Rajamani, Richard T Sayre
Jul 28, 2006·Letters in Applied Microbiology·B Austin, X-H Zhang
Jul 13, 2006·Journal of Applied Microbiology·T NakayamaM Matsumura
Apr 24, 2007·Journal of Applied Microbiology·T NakayamaM Matsumura
Feb 7, 2012·Journal of Invertebrate Pathology·Sonia A Soto-RodriguezJesús L Romalde
Mar 30, 2010·Journal of Applied Microbiology·H A D RuwandeepikaI Karunasagar
Aug 5, 2008·Bioorganicheskaia khimiia·N V MozhinaS V Kostrov
Oct 11, 2001·Microbiology and Immunology·K BiS Shinoda
May 23, 2006·Microbiology and Immunology·Zafar SultanSumio Shinoda
May 19, 2020·Marine Life Science & Technology·Xiao-Hua ZhangBrian Austin
Jul 8, 2019·Current Microbiology·Anwesha Ghosh, Punyasloke Bhadury
Sep 27, 2018·Biocontrol Science·Hitomi KuwaharaHiroshi Morita

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.