Purification and characterization of a high molecular weight histone deacetylase complex (HD2) of maize embryos

Biochemistry
Gerald BroschPeter Loidl

Abstract

The dynamic state of core histone acetylation is maintained by histone acetyltransferases and deacetylases. In germinating maize embryos, four nuclear histone deacetylases can be distinguished. From a chromatin fraction prepared at 72 h after start of embryo germination, we have purified the nuclear histone deacetylase HD2 to homogeneity. Using a sequence of chromatographic steps, we achieved the purification of an enzymatically active high molecular weight protein complex with an apparent molecular mass of 400 kDa, as determined by gel filtration chromatography. The purified enzyme was characterized in terms of enzymatic and kinetic properties, and sensitivity to several histone deacetylase inhibitors. In SDS-polyacrylamide gels, HD2 split into three polypeptides of 45, 42, and 39 kDa, suggesting that the native enzyme is a multimer-protein complex. Electrophoresis under nondenaturing conditions in combination with second dimension SDS-gel electrophoresis indicated that all three protein components of the HD2 complex were enzymatically active. Polyclonal antibodies against each of the three polypeptides were raised in rabbits. Each antiserum reacted with all three polypeptides on Western blots, suggesting that p45, p42, and p3...Continue Reading

References

Oct 20, 1995·The Journal of Biological Chemistry·S KleffR Sternglanz
Nov 1, 1994·The Biochemical Journal·M M Sanchez del PinoV Tordera
Apr 8, 1994·Cell·A P Wolffe
Jun 28, 1996·The Journal of Biological Chemistry·A A CarmenM Grunstein
Sep 5, 1996·Biochimica Et Biophysica Acta·T LechnerP Loidl

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Citations

Feb 15, 2013·Plant Cell Reports·Xujun MaChuanping Yang
Mar 1, 2012·Journal of Experimental Botany·Ming LuoKeqiang Wu
Oct 28, 1999·Nucleic Acids Research·A LusserP Loidl
Mar 31, 2015·Journal of Molecular Biology·Christian Edlich-MuthErnest D Laue
Oct 7, 2008·The International Journal of Biochemistry & Cell Biology·Antonello MaiLucia Altucci
May 8, 2007·The International Journal of Biochemistry & Cell Biology·Antonello MaiLucia Altucci
Feb 18, 2005·Medicinal Research Reviews·Antonello MaiRino Ragno
Feb 18, 2010·Medicinal Research Reviews·Eleni Pontiki, Dimitra Hadjipavlou-Litina
Jun 10, 2011·The New Phytologist·Stéphane BourqueDavid Wendehenne
Nov 19, 2013·Plant, Cell & Environment·Vincent GrandperretStéphane Bourque
Oct 30, 2016·Trends in Plant Science·S BourqueV Nicolas-Francès
Oct 3, 2018·Frontiers in Plant Science·Barbara WójcikowskaMałgorzata D Gaj
Sep 19, 1998·Methods : a Companion to Methods in Enzymology·D KölleP Loidl
Sep 29, 2006·Journal of Medicinal Chemistry·Antonello MaiGerald Brosch
May 23, 2006·Journal of Chemical Information and Modeling·Rino RagnoAntonello Mai

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