Purification and characterization of a Kunitz inhibitor from Poincianella pyramidalis with insecticide activity against the Mediterranean flour moth

Pesticide Biochemistry and Physiology
Lays Cordeiro GuimarãesMaria L R Macedo

Abstract

This paper describes the characterization of a trypsin inhibitor from Poincianella pyramidalis seeds (PpyTI). The partial sequencing of PpyTI revealed homology to Kunitz inhibitors, clustered as a member of Family I03 in MEROPS database. PpyTI has a single polypeptide chain of 19,042 Da and presents stability at high temperatures (up to 70 °C) and a wide range of pH. In vitro assays showed that disulfide bridges have an important stabilization role of reactive site in PpyTI, a characteristic shared among several Kunitz inhibitors. Bioassays carried out with the Mediterranean flour moth (Anagasta kuehniella) revealed a significant decrease in both larval weight and survival of PpyTI-fed larvae, besides a larval stage extension. Through biochemical analysis, we demonstrated that the PpyTI insecticide effects were triggered by digestion process commitment, through the inhibition of trypsin and chymotrypsin activities, the major digestive enzymes in this species. The insecticide effects and biochemical characterization of PpyTI encourage further studies using this inhibitor for insect pest control.

References

Jan 5, 1991·Journal of Molecular Biology·S OnestiD M Blow
Feb 22, 2002·Biochemical and Biophysical Research Communications·Maria do Socorro M CavalcantiClaudio A M Sampaio
Dec 31, 2003·Phytochemistry·Octávio L FrancoMaria Fátima Grossi-de-Sá
Aug 27, 2004·The Protein Journal·Viviane Alves GarciaMaria Lígia Rodrigues Macedo
Nov 11, 2005·The FEBS Journal·John T Christeller
Dec 17, 2005·Journal of Ethnopharmacology·Maria Raquel Ferreira de LimaNicole Moreau
Mar 25, 2006·Planta medica·Joana Tomomi SumikawaMaria Luiza Oliva
Jul 19, 2006·International Journal of Biological Macromolecules·Adriana M NakahataMaria Luiza V Oliva
Mar 25, 2008·Biochimica Et Biophysica Acta·M H Lingaraju, Lalitha R Gowda
Mar 20, 1947·The Journal of General Physiology·M Kunitz
Dec 1, 1946·Proceedings of the Society for Experimental Biology and Medicine·D E BOWMAN
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·K M DunseM A Anderson
Nov 26, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Joabe Gomes de MeloUlysses Paulino de Albuquerque
Mar 23, 2011·Current Protein & Peptide Science·Maria Luiza Vilela OlivaMisako Uemura Sampaio
Nov 17, 2011·Nucleic Acids Research·Neil D RawlingsAlex Bateman
Mar 8, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Caio Fernando Ramalho de OliveiraMaria Ligia Rodrigues Macedo
May 18, 2013·Journal of Proteome Research·Adrian GuthalsNuno Bandeira
Jun 19, 2013·Plant Physiology and Biochemistry : PPB·Ana C B CruzElizeu A Santos
Dec 18, 2013·The International Journal of Biochemistry & Cell Biology·Zongwei LiJianguo Li

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Citations

Jul 26, 2018·Frontiers in Plant Science·Ana ArnaizM E Santamaria
Feb 2, 2020·Plant Science : an International Journal of Experimental Plant Biology·Juliana CotabarrenWalter David Obregón
Jan 23, 2021·International Journal of Molecular Sciences·Mohamed Amine JmelMichalis Kotsyfakis
Dec 15, 2017·Journal of Agricultural and Food Chemistry·Suellen Rodrigues RamalhoMaria Lígia Rodrigues Macedo
Oct 17, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Karina Margareti Alencar de BarrosMaria Lígia Rodrigues Macedo

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