Purification and characterization of a Kunitz inhibitor from Poincianella pyramidalis with insecticide activity against the Mediterranean flour moth

Pesticide Biochemistry and Physiology
Lays Cordeiro GuimarãesMaria L R Macedo


This paper describes the characterization of a trypsin inhibitor from Poincianella pyramidalis seeds (PpyTI). The partial sequencing of PpyTI revealed homology to Kunitz inhibitors, clustered as a member of Family I03 in MEROPS database. PpyTI has a single polypeptide chain of 19,042 Da and presents stability at high temperatures (up to 70 °C) and a wide range of pH. In vitro assays showed that disulfide bridges have an important stabilization role of reactive site in PpyTI, a characteristic shared among several Kunitz inhibitors. Bioassays carried out with the Mediterranean flour moth (Anagasta kuehniella) revealed a significant decrease in both larval weight and survival of PpyTI-fed larvae, besides a larval stage extension. Through biochemical analysis, we demonstrated that the PpyTI insecticide effects were triggered by digestion process commitment, through the inhibition of trypsin and chymotrypsin activities, the major digestive enzymes in this species. The insecticide effects and biochemical characterization of PpyTI encourage further studies using this inhibitor for insect pest control.


Jan 5, 1991·Journal of Molecular Biology·S OnestiD M Blow
Feb 22, 2002·Biochemical and Biophysical Research Communications·Maria do Socorro M CavalcantiClaudio A M Sampaio
Dec 31, 2003·Phytochemistry·Octávio L FrancoMaria Fátima Grossi-de-Sá
Aug 27, 2004·The Protein Journal·Viviane Alves GarciaMaria Lígia Rodrigues Macedo
Nov 11, 2005·The FEBS Journal·John T Christeller
Dec 17, 2005·Journal of Ethnopharmacology·Maria Raquel Ferreira de LimaNicole Moreau
Mar 25, 2006·Planta medica·Joana Tomomi SumikawaMaria Luiza Oliva
Jul 19, 2006·International Journal of Biological Macromolecules·Adriana M NakahataMaria Luiza V Oliva
Mar 25, 2008·Biochimica Et Biophysica Acta·M H Lingaraju, Lalitha R Gowda
Mar 20, 1947·The Journal of General Physiology·M Kunitz
Dec 1, 1946·Proceedings of the Society for Experimental Biology and Medicine·D E BOWMAN
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·K M DunseM A Anderson
Nov 26, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Joabe Gomes de MeloUlysses Paulino de Albuquerque
Mar 23, 2011·Current Protein & Peptide Science·Maria Luiza Vilela OlivaMisako Uemura Sampaio
Nov 17, 2011·Nucleic Acids Research·Neil D RawlingsAlex Bateman
Mar 8, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Caio Fernando Ramalho de OliveiraMaria Ligia Rodrigues Macedo
May 18, 2013·Journal of Proteome Research·Adrian GuthalsNuno Bandeira
Jun 19, 2013·Plant Physiology and Biochemistry : PPB·Ana C B CruzElizeu A Santos
Dec 18, 2013·The International Journal of Biochemistry & Cell Biology·Zongwei LiJianguo Li

❮ Previous
Next ❯


Jul 26, 2018·Frontiers in Plant Science·Ana ArnaizM E Santamaria
Feb 2, 2020·Plant Science : an International Journal of Experimental Plant Biology·Juliana CotabarrenWalter David Obregón
Jan 23, 2021·International Journal of Molecular Sciences·Mohamed Amine JmelMichalis Kotsyfakis
Dec 15, 2017·Journal of Agricultural and Food Chemistry·Suellen Rodrigues RamalhoMaria Lígia Rodrigues Macedo
Oct 17, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Karina Margareti Alencar de BarrosMaria Lígia Rodrigues Macedo

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.