PMID: 39618Aug 30, 1979

Purification and characterization of a long-chain acyl-CoA hydrolase from rat liver microsomes

Biochimica Et Biophysica Acta
R K Berge

Abstract

A long-chain acyl-CoA hydrolase from rat liver microsomes has been purified by solvent extraction and gel chromatography to homogeneity as judged by polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate. The enzyme was a monomer of molecular weight 59 000. In a sucrose gradient it sedimented at 4.3 S. The isoelectric point, pI was 6.9, and the Stokes radius was approx. 31 A. The enzyme hydrolyzed long-chain fatty acyl-CoA (C7--C18) with maximum activity for palmitoyl-CoA. Bovine serum albumin activation of the enzyme was related to the ratio acyl-CoA/bovine serum albumin, and at high ratios, acyl-CoA inhibited the enzyme activity. Disregarding the substrate inhibition, an apparent Km of 65 nmol/mg protein or 1-10(-7) M and a V of 750 nmol/mg protein per min were calculated. The enzyme was inhibited by p-hydroxymercuribenzoate and N-ethylmaleimide. Reactivation by means of dithiothreitol was not complete.

References

Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S SmithK N Dileepan

Citations

Jun 9, 1993·Molecular and Cellular Biochemistry·J KnudsenK Kristiansen
Feb 4, 1992·Biochemical Pharmacology·R M SánchezJ C Laguna
Aug 30, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·R J WandersR B Schutgens
Jan 1, 1988·The International Journal of Biochemistry·K H MooreF P Schreefel
Feb 1, 1994·The International Journal of Biochemistry·S O ShinY Yokota
Dec 21, 2002·Pharmacology & Therapeutics·Riad Qanbar, Michel Bouvier
Jun 1, 1995·European Journal of Biochemistry·L T SvenssonS E Alexson
Aug 1, 1990·Biochemical Pharmacology·D H KimW B Jakoby
Apr 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·M A DiczfalusyS E Alexson
Feb 1, 2002·Current Protocols in Toxicology·Masakiyo Hosokawa, Tetsuo Satoh
Jun 26, 1999·The Journal of Biological Chemistry·V PouponA Benmerah
Aug 1, 1997·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A GarrasR K Berge
May 3, 1996·The Journal of Biological Chemistry·C G BroustasA K Hajra

Related Concepts

Metazoa
Cations
Enzyme Activation
Hydrogen-Ion Concentration
Microsomes, Liver
Osmolality
Thioesterase I
Serum Albumin, Bovine
Thiolester Hydrolases
Rats, Laboratory

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