Purification and characterization of a major endonuclease from rat liver nuclei

Biochemistry
G A CordisM P Deutscher

Abstract

A major endonuclease has been purified approximately 800-fold from rat liver nuclei using poly(A) as substrate. The enzyme had a molecular weight of about 50,000, and active fractions were obtained which contained no nucleic acid. Enzymatic activity was optimal between pH 6 and 7 and was totally dependent on the presence of a divalent cation. The reaction was inhibited by high ionic strength, polydextran sulfate, heparin, and sodium pyrophosphate. The purified enzyme readily hydrolyzed poly(A), poly(U), poly(C), and denatured DNA, whereas poly(G) was not degraded, and transfer RNA, ribosomal RNA, and native DNA were hydrolyzed only at relatively slow rates. These data suggest that the enzyme may be specific for single-stranded polynucleotides. The purified enzyme was essentially devoid of exonuclease activity, and the products of exhaustive endonuclease digestion of poly(A) were small oligonucleotides terminated with a 5'-phosphoryl group. Evidence was obtained that this endonuclease is localized in the nucleoplasm. Possible functions for this activity are discussed.

References

Sep 7, 1973·Biochimica Et Biophysica Acta·A W PrestaykoH Busch
Sep 28, 1973·Science·J E DarnellG R Molloy
Jan 1, 1973·Annual Review of Biochemistry·R A Weinberg
May 15, 1974·European Journal of Biochemistry·A BoctorW Szer
Jan 25, 1974·Journal of Molecular Biology·R P PerryJ LaTorre
May 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·H M Lazarus, M B Sporin
Apr 4, 1969·Analytical Biochemistry·K T SeinD Kanazir
Mar 1, 1966·The Biochemical Journal·P J Curtis, R M Smellie
Dec 1, 1966·Analytical Biochemistry·E Ambellan, V P Hollander
Feb 1, 1972·FEBS Letters·Marc Edouard Mirault, Klaus Scherrer
Aug 1, 1956·Experimental Cell Research·J CHAUVEAUC ROUILLER
Mar 12, 1965·Science·R E THACH, P DOTY

Citations

Aug 1, 1978·Journal of Steroid Biochemistry·R F Cox
Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K W DawesW E Müller
Dec 18, 2001·FEMS Microbiology Reviews·E S Rangarajan, Vepatu Shankar
Jun 3, 1998·The International Journal of Biochemistry & Cell Biology·H J Kwon, D S Kim
Jan 15, 2005·Cancer Science·Minyi ShiToru Takeuchi
Oct 1, 1978·European Journal of Biochemistry·J Sebastián, C F Heredia
Apr 1, 1981·European Journal of Biochemistry·C DenoyaJ L La Torre
Aug 1, 1979·Journal of Neurochemistry·M E Ittel, P Mandel
Feb 15, 2003·FEMS Microbiology Reviews·Neelam A Desai, Vepatu Shankar
Jun 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T Patel, G J Gores
Jun 29, 2011·Critical Reviews in Microbiology·Amruta Pramod Joshi, Sumedha Sharad Deshmukh
Jul 3, 2020·The Journal of Cell Biology·Michelle Grace AcobaSteven M Claypool
Jan 29, 2013·International Journal of Molecular Sciences·Zvezdan Pavlovic, Marica Bakovic

Related Concepts

Metazoa
Cell Nucleus
Chromatography, DEAE-Cellulose
Ion-Exchange Chromatography Procedure
Alkaline DNase
Endonuclease
Hydrogen-Ion Concentration
Liver
Magnesium
Electron Microscopy

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