Purification and characterization of a new family 45 endoglucanase, STCE1, from Staphylotrichum coccosporum and its overproduction in Humicola insolens.

Applied and Environmental Microbiology
Jinichiro KogaToshiaki Kono

Abstract

In the detergent industry, fungal endoglucanases have been used to release microfibrils (defibrillation) from the surface of dyed cellulosic fabrics to enhance color brightness. Although endoglucanases for laundry use must have various properties, such as a neutral or alkaline optimum pH, resistance to anionic surfactants and oxidizing agents (main components in detergents), and high defibrillation activity, all-purpose endoglucanases have not been obtained yet. As a result of screening of endoglucanases, a new family 45 endoglucanase (family 45 glycoside hydrolase), designated STCE1, was obtained and purified to apparent homogeneity from the culture supernatant of Staphylotrichum coccosporum NBRC 31817. The molecular mass of STCE1 was 49 kDa. The optimum pH for the carboxymethyl cellulase activity of STCE1 was 6.0, and the optimum temperature was 60 degrees C. STCE1 was highly resistant to an anionic surfactant and an oxidizing agent. Furthermore, the defibrillation activities on dyed cotton and lyocell fabrics of STCE1 were higher than those of the other representative endoglucanases tested. These results indicate that STCE1 is an all-purpose enzyme for laundry use. A gene encoding STCE1, designated the stce1 gene, was cloned...Continue Reading

References

Mar 14, 1978·Biochimica Et Biophysica Acta·S P Shoemaker, R D Brown
Feb 1, 1992·Biochemical Society Transactions·P BéguinM K Daniel
Feb 1, 1992·Biochemical Society Transactions·T M Wood
Sep 1, 1989·Gene·B HenrissatJ P Mornon
Sep 23, 1993·Nature·G J DaviesM Schülein
Sep 24, 1999·Protein Science : a Publication of the Protein Society·D E OtzenM Schülein
Feb 20, 2003·Journal of Bacteriology·Tatsuki MoriyaToshiaki Kono
Mar 1, 1997·Journal of Insect Physiology·M SLAYTORT INOUE
Oct 11, 2003·Biotechnology Advances·M K Bhat
Jan 1, 1997·Biotechnology Advances·M K Bhat, S Bhat
Jun 25, 2004·The Biochemical Journal·Alisdair B BorastonGideon J Davies
Nov 27, 2004·Bioscience, Biotechnology, and Biochemistry·Atsushi ShimonakaToshiaki Kono
Jun 24, 2005·Bioscience, Biotechnology, and Biochemistry·Yuko BabaToshiaki Kono
Apr 26, 2006·Bioscience, Biotechnology, and Biochemistry·Atsushi ShimonakaToshiaki Kono

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Citations

Jun 21, 2011·International Journal of Molecular Sciences·Xin JinLi-Ming Xia
Feb 11, 2016·Bioprocess and Biosystems Engineering·Ines BenhmadAli Gargouri
Nov 14, 2018·Journal of Basic Microbiology·Francois N Niyonzima
Jul 13, 2018·Microorganisms·Juan-José Escuder-RodríguezMaría-Isabel González-Siso
Nov 14, 2020·Folia Microbiologica·Francois N Niyonzima
Jan 30, 2015·Chemical Reviews·Christina M PayneGregg T Beckham

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