Purification and characterization of a novel cold-adapted phytase from Rhodotorula mucilaginosa strain JMUY14 isolated from Antarctic

Journal of Basic Microbiology
Peng YuJing-Wen Liu

Abstract

A yeast producing a cold-adapted phytase was isolated from Antarctic deep-sea sediment and identified as a Rhodotorula mucilaginosa strain JMUY14 of basidiomycetous yeasts. It was cultured in fermentation optimized by a response surface methodology based on the Box-Behnken design. The maximum activity of phytase reached 205.447 U ml(-1), which was close to the predicted value of 201.948 U ml(-1) and approximately 3.4 times higher than its initial activity. The extracellular phytase was purified by 15.2-fold to homogeneity with a specific activity of 31,635 U mg(-1) by (NH4 )2 SO4 precipitation, and a combination of DEAE Sepharose Fast Flow, SP Sepharose Fast Flow, and Sephadex G-100. The molecular weight of the purified enzyme was estimated to be 63 kDa and its pI was 4.33. Its optimal temperature and pH were 50 °C and 5.0, respectively. Its activity was 85% at 37 °C, and showed good stability at pH 3.0 ∼ 7.0. When compared with mesophilic counterparts, the phytase not only exhibited a higher activity during 20 ∼ 30 °C but also had a low Km (247 µM) and high kcat (1394 s(-1)). The phytase activity was slightly stimulated in the presence of Mg(2+), Fe(2+), Fe(3+), K(+), Na(+), Ca(2+), EDTA, and EGTA and moderately inhibited by C...Continue Reading

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Citations

Dec 13, 2017·Critical Reviews in Biotechnology·Alysson Wagner Fernandes DuarteLara Durães Sette
Dec 2, 2017·Environmental Microbiology Reports·Kristie TannerManuel Porcar
Sep 27, 2018·Current Protein & Peptide Science·Jean-Étienne R L Morlighem, Gandhi Radis-Baptista
Sep 25, 2019·Marine Drugs·Stefano BrunoCinzia Verde
Feb 10, 2020·International Journal of Biological Macromolecules·Muhammad Bilal, Hafiz M N Iqbal
Jun 3, 2021·Journal of Fungi·Stefano VarrellaCinzia Corinaldesi
Jun 11, 2021·Frontiers in Bioengineering and Biotechnology·Claudia CapusoniConcetta Compagno

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