PMID: 3745139Jun 1, 1986Paper

Purification and characterization of a novel alpha-mannosidase from Aspergillus saitoi

Journal of Biochemistry
J Amano, A Kobata

Abstract

An alpha-mannosidase differing from 1,2-alpha-mannosidase was found to occur in Aspergillus saitoi. By a series of column chromatographies the enzyme was purified up to 1,000-fold, and its properties were studied in detail. The enzyme preparation, which was practically free from other exoglycosidases, showed a pH optimum of 5.0. In contrast to 1,2-alpha-mannosidase, the enzyme was strongly activated by Ca2+ ions. p-Nitrophenyl alpha-mannopyranoside was not hydrolyzed by the enzyme. Accordingly, the substrate specificity of the new alpha-mannosidase was studied by using a variety of tritium-labeled oligosaccharides. Studies with linear oligosaccharides revealed that the enzyme cleaves the Man alpha 1----3Man linkage more than 10 times faster than the Man alpha 1----6Man and the Man alpha 1----2Man linkages. Furthermore, it cleaves the Man alpha 1----6Man linkage of the Man alpha 1----6(Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4GlcNAcOT only after its Man alpha 1----3 residue is removed. Because of this specificity, the enzyme can be used as an effective reagent to discriminate R----Man alpha 1----6(Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4(+/- Fuc alpha 1----6)GlcNAcOT from its isomeric counterparts, Man alpha 1...Continue Reading

Citations

Oct 15, 1992·European Journal of Biochemistry·A Kobata
Feb 17, 2001·European Journal of Biochemistry·O FunatsuK Furukawa
Jul 22, 2009·Current Genetics·Jie ZhouGuo-dong Lu
Aug 2, 2001·Glycobiology·A Kobata
Oct 16, 1987·Journal of Chromatography·H HaradaF Koyama
Jul 19, 1995·Biochimica Et Biophysica Acta·S M GaikwadM I Khan
Dec 1, 1991·Archives of Biochemistry and Biophysics·M TandaiA Kobata
Feb 22, 2005·Carbohydrate Research·Vasileios I AthanasopoulosRobert A Rastall
Dec 1, 1994·Journal of Pharmaceutical Sciences·M WeitzhandlerN Avdalovic

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