PMID: 7541790Feb 1, 1995Paper

Purification and characterization of a novel glycoprotein which has significant homology to heavy chains of inter-alpha-trypsin inhibitor family from human plasma

Journal of Biochemistry
N H Choi-MiuraM Tomita

Abstract

Plasmapheresis with a dextran sulfate column is a treatment for patients with hypercholesteremia. When proteins bound to the column during the treatment were fractionated to prepare some known proteins, we found a 57 kDa glycoprotein designated GP57 which showed a new N-terminal amino acid sequence. Western-blot analysis of human plasma revealed that only a 120 kDa protein, GP120, reacted with anti-GP57 antibody. Since GP120 and GP57 had an identical N-terminal amino acid sequence, GP120 is probably the intact form of GP57. The isoelectric point of GP120 was 6.8. N-Glycanase treatment decreased the molecular weight of GP120 by 15 kDa. Neuraminidase and O-glycanase, however, did not affect the molecular weight. Amino acid sequence analyses of the lysylendopeptidase digest of GP120 revealed significant homology to the heavy chains of inter-alpha-trypsin inhibitor (ITI) family. Since GP120 showed no bikunin sequence, and chondroitinase treatment and alkaline treatment of GP120 did not affect its molecular weight, we concluded that GP120 was not a complex with bikunin. We designated GP120 as IHRP (ITI heavy chain-related protein).

Citations

Feb 26, 2013·Journal of Neurology·Hirotaka TanakaHideaki Hara
Mar 31, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Emilia CaputoBrian M Martin
Nov 25, 2004·Progress in Biophysics and Molecular Biology·Paul A NguewaJosé M Pérez
Feb 24, 2001·Biological & Pharmaceutical Bulletin·N H Choi-MiuraM Tomita
Mar 21, 2001·Biological & Pharmaceutical Bulletin·N H Choi-Miura
Mar 21, 2001·Biological & Pharmaceutical Bulletin·N H Choi-MiuraM Tomita
May 31, 2001·Biological & Pharmaceutical Bulletin·N H Choi-MiuraM Tomita
Sep 9, 1999·The Journal of Cell Biology·V A KickhoeferL H Rome
Nov 11, 2008·Connective Tissue Research·Lisheng Zhuo, Koji Kimata
Jun 3, 2014·Journal of Proteome Research·Kevin Brown ChandlerRadoslav Goldman
Apr 6, 2006·Proteomics·Djuro JosicDouglas C Hixson
Oct 3, 1998·The Journal of Biological Chemistry·M Diarra-MehrpourJ P Martin
Feb 19, 1998·Journal of Biomedical Materials Research·M LückR H Müller
May 20, 2008·Journal of Chromatography. a·Spomenka KovacDjuro Josic

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