PMID: 6978519Jan 1, 1982Paper

Purification and characterization of a nuclear SS-B antigen

Scandinavian Journal of Immunology
A M TeppoO Wegelius

Abstract

A nuclear SS-B antigen was isolated from a saline extract of acetone powder of rabbit thymus by precipitation with ammonium sulphate, affinity chromatography with Blue Sepharose CL-6B, and preparative agarose gel electrophoresis. The mol. wt of the antigen was 68,000. Its electrophoretic mobility was similar to that of pre-albumin, and the iso-electric point was around pH 4.0. The main amino acids of the antigen were glutamic acid, leucine, lysine and alanine. Both histidine and tyrosine were also found. The purified antigen precipitated with anti-SS-B sera but not with any other reference antisera. It resembled La and Ha antigens in susceptibility to proteolytic and nucleolytic enzymes and to heat. The purified SS-B antigen, however, had a higher molecular weight than did the Ha and La antigens. The molecule could not be split into subunits with mercaptoethanol or acid. Counter-electrophoresis showed antibodies to the SS-B antigen in sera from patients with rheumatic diseases, including rheumatoid arthritis, systemic lupus erythematosus and Sjögren's syndrome, but not in amy of the control sera.

References

Sep 1, 1977·Arthritis and Rheumatism·E M TanE V Barnett
Jan 1, 1977·The Journal of Clinical Investigation·J F WolfeG C Sharp
May 1, 1975·The Journal of Clinical Investigation·M A Alspaugh, E M Tan
Oct 1, 1975·Annals of Internal Medicine·D D NotmanE M Tan
Mar 1, 1971·Immunochemistry·M MorrisonR G Webster
Mar 1, 1980·Arthritis and Rheumatism·E ScopelitisM A Alspaugh
Aug 26, 1961·Lancet·J R ANDERSONW F KINNEAR

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Citations

May 30, 2019·Frontiers in Neurology·Bruno StuhlmüllerEugen Feist
Mar 1, 1985·Molecular and Cellular Biology·A M FrancoeurM B Mathews

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