PMID: 9443372Aug 1, 1997Paper

Purification and characterization of a protease degrading 30 kDa yolk proteins of the silkworm, Bombyx mori

Insect Biochemistry and Molecular Biology
N Maki, O Yamashita

Abstract

The second major yolk proteins, 30 kDa proteins (30kPs) of the silkworm, Bombyx mori, which have been provided during oogenesis, are kept continuously unused during embryogenesis and are utilized just before larval hatching. The crude extracts of newly hatched larvae cleaved 30kPs in an in vitro incubation system. A protease was highly purified from newly hatched larvae using ammonium sulfate precipitation, gel filtration and ionic exchange chromatography, and non-denaturing-polyacrylamide gel electrophoresis (ND-PAGE). The protease shared the NH2-terminal amino acid sequence conserved in many serine proteases, and the apparent molecular mass was estimated to be approximately 600 kDa by gel filtration column chromatography. The enzymatic activity was strongly inhibited by elastatinal and diisopropyl fluorophosphate (DFP), indicating that this protease is an elastase-like serine protease. The protease selectively hydrolysed 30kP-1 and 30kP-4 between Ser6 and Ala7, but could not attack other 30kPs such as 30kP-2, 30kP-3 and 30kP-5. Consequently, the protease characterized in the present study is a unique protease which may be specialized for the selective degradation of yolk proteins in silkworm eggs.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1977·The Biochemical Journal·A M Blow
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M IkedaO Yamashita
Oct 5, 1990·European Journal of Biochemistry·T Kageyama, S Y Takahashi
Feb 28, 1988·Biochimica Et Biophysica Acta·N SakaiS Tomino
Aug 1, 1969·The Biochemical Journal·A S Narayanan, R A Anwar
Oct 1, 1984·The Journal of Experimental Zoology·P D ShirkV J Brookes

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Citations

Dec 28, 1999·Insect Biochemistry and Molecular Biology·C Girard, L Jouanin
Dec 6, 2012·Insect Biochemistry and Molecular Biology·Yan ZhangQingyou Xia
Sep 4, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Andréia EstrelaCarlos Termignoni
Mar 5, 2014·Insect Science·Britto Cathrin PakkianathanMuthukalingan Krishnan

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