PMID: 2511556Nov 25, 1989Paper

Purification and characterization of a protein that binds to the recombination signal sequence of the immunoglobulin J kappa segment

Nucleic Acids Research
Y HamaguchiT Honjo

Abstract

A protein that binds to the recombination signal sequence (RS) of the immunoglobulin J kappa segment was purified almost to homogeneity from the nuclear extract of a murine pre-B cell line 38B9. A similar binding protein was found in lymphoid cell lines but not in non-lymphoid cell lines. The binding activity was associated with a polypeptide with a molecular weight of 60,000. DNase I footprinting analysis demonstrated that this binding protein interacted with the heptamer and several 3' bases close to the heptamer. The Kd value of the J kappa RS binding protein to the J kappa RS was 1 nM. One base substitution in the heptamer of the J kappa RS greatly reduced the affinity of the J kappa RS binding protein. The high specificity of the binding site of the J kappa RS binding protein suggests that this protein may be involved in V-J recombination.

References

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Citations

Jan 1, 1995·Advances in Biophysics·C Oka, M Kawaichi
Sep 1, 1991·Molecular and Cellular Biology·M ShirakataH Sakano
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S R GrossmanE Kieff
Mar 25, 1994·Nucleic Acids Research·T TunM Kawaichi
Jul 13, 2012·Genetics·Iva Greenwald
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·T G DoyleI Greenwald
Oct 30, 2009·Protein Science : a Publication of the Protein Society·David R Friedmann, Rhett A Kovall
Oct 24, 2001·Seminars in Cancer Biology·U Zimber-Strobl, L J Strobl
Mar 6, 2007·Developmental Cell·Joaquín Grego-BessaJosé Luis de la Pompa
Dec 28, 2016·Transcription·Benedetto Daniele GiaimoTilman Borggrefe
Feb 9, 2018·Cellular and Molecular Life Sciences : CMLS·Marie-Chantal GrégoireGuylain Boissonneault
Dec 1, 1994·The Japanese Journal of Human Genetics·M ZhangK Yokoyama
Nov 24, 1999·The Journal of General Virology·K G KrauerT Sculley
May 1, 1997·Molecular and Cellular Biology·S MinoguchiT Honjo
Jun 1, 1995·Journal of Virology·D Marshall, C Sample
Oct 17, 2021·Journal of Cellular and Molecular Medicine·Mengjie LiuWenjuan Wang

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