Purification and characterization of a salt-tolerant cellulase from the mangrove oyster, Crassostrea rivularis

Acta Biochimica Et Biophysica Sinica
Tianchen AnShiming Deng

Abstract

A cellulase with wide range of pH resistance and high salt tolerance was isolated from the digestive gland of the oyster Crassostrea rivularis living in mangrove forests. The 27 kDa cellulase named as CrCel was purified 40.6 folds by anion exchange chromatography and extraction from the gel after non-reducing sodium dodecylsufate-polyacrylamide gel electrophoresis. The specific activity of the purified cellulase was 23.4 U/mg against carboxymethyl cellulose (CMC). The N-terminal amino acid sequence of CrCel was determined to be NQKCQANSRV. CrCel preferably hydrolyzes β-1,4-glucosidic bonds in the amorphous parts of cellulose materials and displays degradation activity toward xylan. The Km and Vmax values of CrCel for CMC were determined to be 2.1% ± 0.4% and 73.5 ± 3.3 U mg(-1), respectively. The optimal pH value and temperature of CrCel were 5.5 and 40°C, respectively. The enzyme was stable in a wide range of pH, retaining over 60% activity after incubation for 80 min in the pH range of 3.0-9.0. In addition, CrCel showed remarkable tolerance to salt and remained active at high NaCl concentrations, but also retained over 70% activity after incubation in 0.5-2 M NaCl for up to 24 h. On the basis of the N-terminal sequence alignm...Continue Reading

References

Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·G SmantJ Bakker
Aug 5, 1998·Nature·H WatanabeN Lo
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
Feb 13, 2003·European Journal of Biochemistry·Ken-ichi SuzukiKiyoyoshi Nishita
Oct 11, 2005·Acta Biochimica Et Biophysica Sinica·Yan-Hong LiFu-Kun Zhao
Dec 13, 2006·Cellular and Molecular Life Sciences : CMLS·Q-G XueJ F La Peyre
Oct 24, 2007·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Rui GuoFu-Kun Zhao
Apr 26, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kentaro SakamotoHaruhiko Toyohara
May 21, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kentaro Sakamoto, Haruhiko Toyohara
Sep 17, 2009·Annual Review of Entomology·Hirofumi Watanabe, Gaku Tokuda
Feb 26, 2010·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Ju L GanWen G Cai
Apr 16, 2010·Applied Microbiology and Biotechnology·Zhaoming GaoXun Xu
Dec 15, 2010·Annual Review of Marine Science·I C FellerM C Ball
May 3, 2011·Current Opinion in Microbiology·David B Wilson
Jun 15, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Stuart M Linton, Alicia J Shirley
May 24, 2012·Insect Biochemistry and Molecular Biology·Chia-Jung ChangYu-Chan Chao
Sep 18, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Akihiko TsujiKeizo Yuasa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.