PMID: 6406222May 16, 1983Paper

Purification and characterization of a secreted protease from Tetrahymena pyriformis

European Journal of Biochemistry
Y BannoY Nozawa

Abstract

A simple major protease, secreted into the medium during growth of Tetrahymena pyriformis strain W, has been purified about 4000-fold by (NH4)2SO4 precipitation, ion-exchange chromatography, gel filtration and affinity chromatography on organomercurial-Sepharose. The purified protease was homogeneous as judged by polyacrylamide gel electrophoresis and was a monomeric protein with a molecular weight of 22 000-23 000. Amino acid analysis showed that the enzyme was rich in acidic amino acids. In addition, the purified Tetrahymena protease consists of multiple forms with isoelectric point between pH 5.3 and 6.3. Optimum activity of the purified enzyme was in the pH range 6.5-8.0 with alpha-N-benzoyl-DL-arginine-p-nitroanilide and with azocasein, while it was in the lower pH range (4.5-5.5) for denatured hemoglobins. The purified enzyme was inhibited by compounds effective against thiol proteases. Leupeptin and chymostatin were potent inhibitors but pepstatin was without effect. This enzyme is similar to cathepsin B and appears to be a major proteolytic enzyme in Tetrahymena.

References

Feb 1, 1976·Archives of Biochemistry and Biophysics·M R LevyC L McConkey
Dec 1, 1979·European Journal of Biochemistry·T TowatariN Katunuma
Jun 1, 1979·European Journal of Biochemistry·Y BannoN Katunuma
Jan 1, 1974·Methods in Enzymology·L A Sluyterman, J Wijdenes
May 1, 1972·Analytical Biochemistry·A J Barrett
Dec 1, 1973·The Journal of Antibiotics·H UmezawaT Takeuchi
Feb 1, 1974·The Biochemical Journal·A A SwansonS S Spicer
Apr 1, 1970·The Biochemical Journal·A J Barrett
May 17, 1982·European Journal of Biochemistry·R Perona, C G Vallejo
Apr 1, 1956·Archives of Biochemistry and Biophysics·T VISWANATHA, I E LIENER
Sep 20, 1938·The Journal of General Physiology·M L Anson

❮ Previous
Next ❯

Citations

Aug 22, 2002·The Journal of Eukaryotic Microbiology·Shulin WangYoshinori Nozawa
Jul 20, 2002·The International Journal of Biochemistry & Cell Biology·Kiyoshi Furuhashi
Nov 1, 1992·The Journal of protozoology·J W StrausM T Finch
Sep 21, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sang Uk LimHyung Ho Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.