PMID: 2495263Apr 1, 1989Paper

Purification and characterization of a soybean flour-induced cytochrome P-450 from Streptomyces griseus

Journal of Bacteriology
M K TrowerD P O'Keefe

Abstract

A soybean flour-induced, soluble cytochrome P-450 (P-450soy) was purified 130-fold to homogeneity from Streptomyces griseus. Native cytochrome P-450soy is a single polypeptide, with a molecular weight of 47,500, in association with one ferriprotoporphyrin IX prosthetic group. Oxidized P-450soy exhibited visible absorption maxima at 394, 514, and 646 nm, characteristic of a high-spin cytochrome P-450. The CO-reduced difference spectrum of P-450soy had a Soret maximum at 448 nm. When reconstituted with spinach ferredoxin and spinach ferredoxin:NADP+ oxidoreductase, purified cytochrome P-450soy catalyzed the NADPH-dependent oxidation of the xenobiotic substrates precocene II and 7-ethoxycoumarin. In vitro proteolysis of cytochrome P-450soy generated a stable and catalytically active cytochrome P-450, designated P-450soy delta.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1978·Methods in Enzymology·D H O'KeeffeJ A Peterson
Jan 1, 1978·Methods in Enzymology·C A Appleby
Dec 15, 1986·Biochemical and Biophysical Research Communications·F S Sariaslani, D A Kunz
Aug 1, 1987·Applied and Environmental Microbiology·F S SariaslaniD W Ovenall
Dec 1, 1986·Archives of Biochemistry and Biophysics·C J PattenC S Yang
Feb 1, 1985·Applied and Environmental Microbiology·F S Sariaslani, J P Rosazza
Sep 30, 1970·Biochemical and Biophysical Research Communications·K DusI C Gunsalus
Jun 1, 1984·Journal of Medicinal Chemistry·F S SariaslaniJ P Rosazza
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A N Kotake, Y Funae
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Aug 1, 1983·Applied and Environmental Microbiology·F S Sariaslani, J P Rosazza

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Citations

Sep 1, 1991·Molecular Microbiology·D P O'Keefe, P A Harder
Jan 14, 2010·Journal of Biochemistry·Tsuneo Omura
Sep 4, 2012·International Journal of Molecular Sciences·Bin ZhaoDavid C Lamb
Jun 7, 2013·Expert Opinion on Drug Metabolism & Toxicology·David C LambBin Zhao
Jun 1, 1996·Molecular Microbiology·A W Munro, J G Lindsay
Dec 14, 2011·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Tsuneo Omura
Sep 3, 2014·Biotechnology Letters·Cormac D Murphy
Jan 1, 1989·Critical Reviews in Biotechnology·F S Sariaslani
Nov 5, 2008·Archives of Pharmacal Research·H K Kang, S S Lee
Jan 7, 2003·Applied and Environmental Microbiology·Kajari DharJohn P N Rosazza
Oct 12, 2000·Biochemical and Biophysical Research Communications·D C LambS L Kelly
Mar 1, 1996·Microbiological Reviews·G F WhiteE C Tidswell
Mar 1, 1990·Biochimica Et Biophysica Acta·M K TrowerF S Sariaslani
Jun 1, 1990·Journal of Bacteriology·C A OmerD P O'Keefe
Jul 31, 1990·Biochemical and Biophysical Research Communications·F S SariaslaniF G Kitson

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