Purification and characterization of a thermostable keratinolytic serine alkaline proteinase from Streptomyces sp. strain AB1 with high stability in organic solvents

Bioresource Technology
Bassem JaouadiSamir Bejar

Abstract

A keratinolytic alkaline proteinase (KERAB) was isolated from Streptomyces sp. strain AB1. Based on MALDI-TOF mass spectrometry analysis, the purified enzyme is a monomer with a molecular mass of 29850.17Da. The NH(2)-terminal sequence of the enzyme was determined to be TQANPPSWGLDDIDQTAL. This keratinase was completely inhibited by phenylmethanesulfonyl fluoride (PMSF) and diiodopropyl fluorophosphates (DIFP), which suggests that it belongs to the serine protease family. Using keratin azure as a substrate, the optimum pH and temperature values for keratinase activity were pH 11.5 and 75 degrees C, respectively. This keratinase was stable between 30 and 60 degrees C and pH 4 and 11 for 4 and 96 h, respectively, and thermoactivity and thermostability were enhanced in the presence of 5 mM Mg(2+). Its catalytic efficiency was higher than those of SAPB-L31I/T33S/N99Y, nattokinase and subtilisin Carlsberg. KERAB exhibited stability to detergents and high resistance against organic solvents and was able to degrade feathers completely. These properties make KERAB a potential candidate for future applications in detergent formulations, dehairing during leather processing, and non-aqueous peptide biocatalysis.

References

Nov 1, 1979·Analytical Biochemistry·E G DelMarM C Geokas
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·W M AwadT P Toomey
Jun 20, 2002·Applied Microbiology and Biotechnology·R GuptaP Lorenz
Oct 31, 2002·Journal of Industrial Microbiology & Biotechnology·A Riffel, A Brandelli
Aug 7, 2004·Current Opinion in Biotechnology·Karl-Heinz Maurer
Jun 6, 2007·Biochemical and Biophysical Research Communications·John F Hess, Paul G FitzGerald
Sep 7, 2007·Bioresource Technology·Radhika TatineniLakshmi Narasu Mangamoori
Nov 8, 2008·Bioresource Technology·Dastager G SyedDayanand Agasar
Apr 25, 2009·Acta Microbiologica Et Immunologica Hungarica·J P EssienA Umoiyoho
Dec 30, 2009·Applied Microbiology and Biotechnology·Adriano BrandelliAlessandro Riffel

❮ Previous
Next ❯

Citations

Mar 25, 2014·Colloids and Surfaces. B, Biointerfaces·Yanina N MartínezGuillermo R Castro
Aug 21, 2013·Applied Microbiology and Biotechnology·Maachi KataokaKunihiko Watanabe
Jan 27, 2016·International Journal of Biological Macromolecules·Khelifa BouacemBassem Jaouadi
Dec 22, 2015·Carbohydrate Research·Bouanane-Darenfed AmelFardeau Marie-Laure
Oct 19, 2013·Journal of Basic Microbiology·Krish SuthindhiranApurva Prasar
Jun 19, 2015·International Journal of Biological Macromolecules·Nadia Zaraî JaouadiBassem Jaouadi
Jun 10, 2015·International Journal of Biological Macromolecules·Mouna Ben ElhoulBassem Jaouadi
May 22, 2013·BioMed Research International·Divya PrakashBalasaheb Kapadnis
May 24, 2015·World Journal of Microbiology & Biotechnology·Souraya Boulkour TouiouiBassem Jaouadi
Jun 4, 2013·International Journal of Biological Macromolecules·Bassem JaouadiSamir Bejar
Aug 31, 2013·BioMed Research International·Panchanathan ManivasaganSe-Kwon Kim
Jun 15, 2016·International Journal of Biological Macromolecules·Maroua Omrane BenmradBassem Jaouadi
Jul 9, 2016·International Journal of Biological Macromolecules·Mouna Ben ElhoulBassem Jaouadi
Oct 29, 2015·Journal of Bioscience and Bioengineering·Yayue WangSong Xue
Mar 5, 2018·Applied Microbiology and Biotechnology·Jelena SpasicJasmina Nikodinovic-Runic
Aug 27, 2019·Frontiers in Bioengineering and Biotechnology·Sandeep Kaur SagguPrakash Chandra Mishra
Dec 24, 2019·Frontiers in Microbiology·Qingxin Li
Apr 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nonso E NnolimUchechukwu U Nwodo
Nov 8, 2020·Environmental Science and Pollution Research International·Mouna Ben ElhoulBassem Jaouadi
Nov 17, 2020·The Science of the Total Environment·Dingrong KangSøren J Sørensen
Aug 24, 2019·Heliyon·Fatema MatkawalaAnand Nighojkar
Jan 5, 2021·Frontiers in Microbiology·Nonso E NnolimUchechukwu U Nwodo
Mar 21, 2020·International Journal of Biological Macromolecules·Mohamed A HassanEman Abbas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.