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Purification and characterization of a collagenase extracted from rabbit tumours

The Biochemical Journal

Oct 1, 1975

P A McCroskeryEdward D Harris

PMID: 2161

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Abstract

A collagenase was purified from homogenates of V2 ascites-cell carcinoma growing in rabbit muscle. (NH4)2SO4 precipitation, ion-exchange and gel-filtration chromatography, and affinity chromatography (by using the CB7 CNBr) cleavage fragment of alpha 1(I) collagen linked to agarose) gav...read more

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Metal Antagonists
Collagenase-Like Peptidase
Centrifugation, Density Gradient
Calcium
Hydrogen-Ion Concentration
SDS-PAGE
Tumor Lysis Syndrome
Synovial Membrane
Neoplasms, Experimental
Immunodiffusion Measurement
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  • Purification and characterization of a collagenase extracted from rabbit tumours

    The Biochemical Journal

    Oct 1, 1975

    P A McCroskeryEdward D Harris

    PMID: 2161

    DOI:

    Abstract

    A collagenase was purified from homogenates of V2 ascites-cell carcinoma growing in rabbit muscle. (NH4)2SO4 precipitation, ion-exchange and gel-filtration chromatography, and affinity chromatography (by using the CB7 CNBr) cleavage fragment of alpha 1(I) collagen linked to agarose) gav...read more

    Mentioned in this Paper

    Metal Antagonists
    Collagenase-Like Peptidase
    Centrifugation, Density Gradient
    Calcium
    Hydrogen-Ion Concentration

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