Abstract
Two novel acharan sulfate lyases (ASL1 and ASL2: no EC number) have been purified from Bacteroides stercoris HJ-15 which was isolated from human intestinal bacteria with glycosaminoglycan (GAG) degrading enzymes. These enzymes were purified to apparent homogeneity by a combination of QAE-cellulose, DEAE-cellulose, carboxymethyl-Sephadex C-50, hydroxyapatite and HiTrap SP Sephadex C-25 column chromatography with the final specific activity of 50.5 and 76.7 micromol.min-1.mg-1, respectively. Both acharan sulfate lyases are single subunits of 83 kDa by SDS/PAGE and gel filtration. ASL1 showed optimal activity at pH 7.2 and 45 degrees C. ASL1 activity was inhibited by Cu2+, Ni2+ and Co2+, but ASL2 activity was inhibited by Cu2+, Ni2+and Pb2. Both enzymes were slightly inhibited by some agents that modify histidine and cysteine residues, but activated by reducing agents such as DL-dithiothreitol and 2-mercaptoethanol. Both purified bacteroidal acharan sulfate lyases acted to the greatest extent on acharan sulfate, and to a lesser extents on heparan sulfate and heparin. They did not act on de-O-sulfated acharan sulfate. These findings suggest that the biochemical properties of these purified acharan sulfate lyases are different from ...Continue Reading
References
Feb 1, 1977·Applied and Environmental Microbiology·A A SalyersT D Wilkins
May 7, 1976·Analytical Biochemistry·M M Bradford
Jan 1, 1992·Advances in Experimental Medicine and Biology·J Folkman, Y Shing
Apr 1, 1991·Physiological Reviews·R L JacksonA D Cardin
May 1, 1986·The Journal of Cell Biology·J J CastellotM J Karnovsky
Aug 15, 1970·Nature·U K Laemmli
Nov 1, 1994·Clinical and Experimental Dermatology·T HiguchiY Miyachi
Nov 1, 1993·Archives of Biochemistry and Biophysics·U R DesaiR J Linhardt
Aug 17, 1993·Biochemistry·U R DesaiR J Linhardt
Jan 15, 1993·The Biochemical Journal·M C Bourin, U Lindahl
Oct 16, 1995·Carbohydrate Research·C C GriffinS E Brown
May 17, 1996·The Journal of Biological Chemistry·Y S KimR J Linhardt
Aug 1, 1996·Applied and Environmental Microbiology·H SuD C Bennett
Aug 23, 1996·Biochemical and Biophysical Research Communications·R GodavartiR Sasisekharan
Apr 16, 1998·Journal of Biochemistry·M WatanabeK Sugahara
Aug 12, 1998·Canadian Journal of Microbiology·M Y AhnY S Kim
Aug 2, 2000·Journal of Biochemistry·B T KimD H Kim
Citations
Aug 2, 2008·Glycoconjugate Journal·Youmie ParkRobert J Linhardt
Nov 3, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J JeongY S Kim
Jun 20, 2002·European Journal of Biochemistry·Sung-Woon HongDong-Hyun Kim
Jul 19, 2003·European Journal of Biochemistry·Sung-Woon HongDong-Hyun Kim
Jul 2, 2009·Journal of Applied Microbiology·Y-J HyunD-H Kim
Dec 21, 2004·Journal of Biochemistry and Molecular Biology·Wan-Seok KimYeong Shik Kim
Jan 30, 2008·Protein Expression and Purification·Kyu-Woong Shim, Dong-Hyun Kim
Jul 1, 2004·Critical Reviews in Biotechnology·P MichaudJ Courtois