Abstract
We have demonstrated recently that phenylazonaphthol (PAN) allergy-induced hyperpigmentation in brownish guinea pig skin is associated with the concomitant appearance of a melanogenic soluble factor(s) that activates the intracellular signal transduction system, including phosphatidylinositol turnover subsequent to ligand-receptor binding in cultured guinea pig melanocytes. In this study we have purified and characterized the PAN-induced melanogenic stimulating factor (PIMSF) that occurs in allergy-associated hyperpigmented skin. By successive column chromatography on TSK 2000SW, Mono Q, and octadecyl-NPR, the PIMSF was purified to homogeneity with a single band of apparent molecular mass of 7.9 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The specific bioactivity of PIMSF increased by 5,195-fold over the original skin homogenate. In cultured guinea pig melanocytes, this purified PIMSF had the potential of activating an intracellular signal transduction system such as inositol 1,4,5-trisphosphate formation and intracellular calcium levels through a pertussis toxin-sensitive G protein-coupled receptor. PIMSF consistently caused a rapid translocation of cytosolic protein kinase C (PKC) to membrane-bound PKC w...Continue Reading
References
Oct 1, 1992·The Journal of Investigative Dermatology·G ImokawaM Okuda
Nov 6, 1990·Biochemistry·E BalentienK Matsushima
Oct 1, 1988·The Journal of Cell Biology·R HalabanJ McGuire
Sep 29, 1989·Biochemical and Biophysical Research Communications·Y YadaY Nozawa
Feb 1, 1989·The Journal of Investigative Dermatology·G Imokawa, T Tejima
Nov 15, 1988·Biochemical and Biophysical Research Communications·M SeishimaY Nozawa
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A AnisowiczR Sager
Dec 16, 1987·Biochemical and Biophysical Research Communications·A WalzM Baggiolini
Apr 1, 1986·The Journal of Investigative Dermatology·J J NordlundL A Rheins
Dec 1, 1987·The Journal of Investigative Dermatology·G Imokawa, M Kawai
Jan 1, 1986·Archives of Dermatological Research·G ImokawaI Motegi
Jun 20, 1985·Nature·A D LusterJ V Ravetch
Aug 15, 1970·Nature·U K Laemmli
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C W CastorD A Walz
Jun 1, 1995·Experimental Dermatology·T J VennerR C Mckenzie
Jul 1, 1995·The Journal of Investigative Dermatology·G ImokawaY Yada
Dec 1, 1993·The Journal of Investigative Dermatology·T KojimaJ T Elder
Jan 1, 1993·The Journal of Investigative Dermatology·G Imokawa, I Motegi
Jan 15, 1996·The Biochemical Journal·G ImokawaN Morisaki
Feb 15, 1996·The Biochemical Journal·G ImokawaM Kimura
Jul 15, 1996·The Journal of Clinical Investigation·R W GrovesT S Kupper
Citations
Sep 6, 2001·Clinics in Dermatology·R WolfY Tüzün
Oct 31, 2008·Plastic and Reconstructive Surgery·Mathias C AustPeter M Vogt
Apr 5, 2001·The Journal of Investigative Dermatology·S KadonoG Imokawa
May 14, 2004·The Journal of Investigative Dermatology·Hideko HattoriGenji Imokawa
Sep 21, 2001·The Journal of Investigative Dermatology·E ShishidoG Imokawa
Mar 16, 2004·Pigment Cell Research·Genji Imokawa
Sep 24, 2010·The Journal of Dermatology·Shigeki InuiSatoshi Itami
Jul 31, 2019·International Journal of Molecular Sciences·Genji Imokawa