Purification and characterization of an allergy-induced melanogenic stimulating factor in brownish guinea pig skin.

The Journal of Biological Chemistry
G ImokawaY Yada

Abstract

We have demonstrated recently that phenylazonaphthol (PAN) allergy-induced hyperpigmentation in brownish guinea pig skin is associated with the concomitant appearance of a melanogenic soluble factor(s) that activates the intracellular signal transduction system, including phosphatidylinositol turnover subsequent to ligand-receptor binding in cultured guinea pig melanocytes. In this study we have purified and characterized the PAN-induced melanogenic stimulating factor (PIMSF) that occurs in allergy-associated hyperpigmented skin. By successive column chromatography on TSK 2000SW, Mono Q, and octadecyl-NPR, the PIMSF was purified to homogeneity with a single band of apparent molecular mass of 7.9 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The specific bioactivity of PIMSF increased by 5,195-fold over the original skin homogenate. In cultured guinea pig melanocytes, this purified PIMSF had the potential of activating an intracellular signal transduction system such as inositol 1,4,5-trisphosphate formation and intracellular calcium levels through a pertussis toxin-sensitive G protein-coupled receptor. PIMSF consistently caused a rapid translocation of cytosolic protein kinase C (PKC) to membrane-bound PKC w...Continue Reading

References

Feb 1, 1989·The Journal of Investigative Dermatology·G Imokawa, T Tejima
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A AnisowiczR Sager
Dec 16, 1987·Biochemical and Biophysical Research Communications·A WalzM Baggiolini
Dec 1, 1987·The Journal of Investigative Dermatology·G Imokawa, M Kawai
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C W CastorD A Walz
Jan 1, 1993·The Journal of Investigative Dermatology·G Imokawa, I Motegi

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Citations

Sep 6, 2001·Clinics in Dermatology·R WolfY Tüzün
Apr 5, 2001·The Journal of Investigative Dermatology·S KadonoG Imokawa
May 14, 2004·The Journal of Investigative Dermatology·Hideko HattoriGenji Imokawa
Jul 31, 2019·International Journal of Molecular Sciences·Genji Imokawa

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