PMID: 9435109Jan 22, 1998Paper

Purification and characterization of an alpha-L-rhamnosidase from Aspergillus niger

FEMS Microbiology Letters
P ManzanaresJ Visser

Abstract

An enzyme with alpha-L-rhamnosidase activity was purified by anion exchange chromatography from an Aspergillus niger commercial preparation. The alpha-L-rhamnosidase was shown to be N-glycosylated, and had a molecular mass of 85 kD on sodium dodecylsulfate-polyacrylamide gel electrophoresis of which approximately 12% was contributed by carbohydrate. The enzyme was optimally active at pH 4.5 and 65 degrees C. When tested towards p-nitrophenyl-alpha-L-rhamnopyranoside it showed Km and Vmax values of 2.9 mM and 20.6 U mg-1, respectively whereas it was inhibited competitively by L-rhamnose (Ki 3.5 mM). Substrate specificity studies showed alpha-L-rhamnosidase to be active both on alpha-1,2 and alpha-1,6 linkages to beta-D-glucose. Moreover, the enzyme was able to release L-rhamnose from geranyl-beta-D-rutinoside and 2-phenylethyl-beta-D-rutinoside.

References

Sep 1, 1985·Analytical Biochemistry·C RomeroJ L Iborra
Dec 1, 1987·Biotechnology Letters·M A SánchezJ L Iborra

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Citations

Aug 3, 2000·Bioscience, Biotechnology, and Biochemistry·T NarikawaT Fujii
Apr 27, 2010·Biodegradation·Sylvain TranchimandGilles Iacazio
Sep 6, 2000·Letters in Applied Microbiology·P ManzanaresD Ramón
Jul 18, 2008·Applied Microbiology and Biotechnology·Takuya KosekiHaruyuki Iefuji
Jan 16, 2016·International Journal of Biological Macromolecules·Lijun LiFeng Chen
Oct 16, 2007·Journal of Molecular Biology·Zhongli CuiKousaku Murata
May 28, 2004·Journal of Biotechnology·Anne Casey, Gary Walsh
Jul 4, 2015·Journal of Food Science and Technology·B S Gunashree, G Venkateswaran
Oct 25, 2013·Preparative Biochemistry & Biotechnology·Pavithra MukundM B Saidutta
May 28, 2011·Journal of Agricultural and Food Chemistry·Lea Dalby-BrownNiels Agerbirk
Jan 8, 2013·Journal of Agricultural and Food Chemistry·YueLong ChenWenJin Su
Feb 7, 2012·Biotechnology Advances·Feng PengRun-Cang Sun
Oct 31, 2012·Journal of Molecular Recognition : JMR·Andreia F M FurtadoMaria H L Ribeiro
May 1, 2012·Applied and Environmental Microbiology·Tingqiang LiuFengxie Jin
Jul 23, 2011·Applied Microbiology and Biotechnology·Fernando SoriaLuiz Bezerra Carvalho
Jun 30, 2010·Journal of Basic Microbiology·Praveen DahiyaNeeraj Dilbaghi
Nov 15, 2011·Applied Microbiology and Biotechnology·Munish Puri
Sep 4, 2020·Critical Reviews in Food Science and Nutrition·Zijian LiangPangzhen Zhang

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