PMID: 702645Oct 1, 1978Paper

Purification and characterization of an early protein (E14K) from adenovirus type 2-infected cells

Journal of Virology
H PerssonL Philipson

Abstract

One adenovirus type 2 (Ad2) early protein, with an apparent molecular weight of 14,000 in sodium dodecyl sulfate-polyacrylamide gels (E14K), was purified to homogeneity. Purification involved fractionation of cytoplasmic extracts, precipitation at low pH, and DEAE-cellulose, phosphocellulose, and hydroxylapatite chromatography. The yield was around 12 microgram of purified protein per 10(9) HeLa cells. The two Ad2 DNA binding proteins with molecular weights of 75,000 and 45,000 (E75K and E45K) were purified by the same procedure. Tryptic peptide analyses indicated that the E14K protein is unrelated to the DNA binding proteins. The purified E14K protein has a high content of basic amino acids and a sedimentation coefficient of 5.5S in the native state, corresponding to a molecular weight of around 95,000. Pulse-chase experiments suggest that the E14K polypeptide is a primary translation product. Immunoprecipitation with a monospecific antiserum against the E14K protein revealed that it is exclusively localized in the cytoplasm of infected cells. E14K started to be synthesized at 2 hpostinfection, with a maximal rate of synthesis at 4 to 6 h postinfection. Immunoprecipitation of cell extracts from four different Ad2-transformed h...Continue Reading

References

Jan 1, 1975·Cold Spring Harbor Symposia on Quantitative Biology·F L GrahamA J Van Der Eb
Dec 1, 1975·Journal of Virology·D F Klessig, C W Anderson
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F Toneguzzo, H P Ghosh
Jan 1, 1975·Cold Spring Harbor Symposia on Quantitative Biology·P A SharpS J Flint
Mar 1, 1976·Journal of Virology·J L Saborio, B Oberg
Mar 15, 1976·Journal of Molecular Biology·U PetterssonL Philipson
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Aug 1, 1973·Journal of Virology·C W AndersonR F Gesteland
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey

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Citations

Apr 23, 2002·Protein Science : a Publication of the Protein Society·Hee-Jung Kim, Mark P Foster
Dec 24, 2003·International Reviews of Immunology·Drew L LichtensteinWilliam S M Wold
Feb 1, 1990·Journal of Virology·A E TollefsonW S Wold
Jun 1, 1982·Journal of Virology·H PerssonL Philipson
Feb 5, 1980·Journal of Molecular Biology·H PerssonL Philipson
Aug 5, 1979·Journal of Molecular Biology·D M FowlkesL Philipson

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