PMID: 2502946Aug 15, 1989Paper

Purification and characterization of an ethanol-induced UDP-glucuronosyltransferase

Archives of Biochemistry and Biophysics
R M Hutabarat, G S Yost

Abstract

A UDP-glucuronosyltransferase (GT) enzyme was isolated from ethanol-induced male New Zealand white rabbit hepatic protein. The animals were pretreated for 2 weeks with 10% ethanol in their drinking water. The GT enzyme was purified by anion-exchange and affinity chromatography and was shown to be homogeneous by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis. The molecular mass of the ethanol-induced UDP-glucuronosyltransferase was determined to be 57,000 Da. Tryptic digests of the ethanol-induced GT and a similarly purified GT from control rabbit liver appeared to be different by HPLC analysis, even though the molecular masses of the enzymes were indistinguishable. Amino acid compositions of the two proteins were different for six amino acids. The apparent Km values for the ethanol-induced GT enzyme for 1-naphthol and morphine as substrates were 43 and 109 microM, respectively. The apparent Vmax values for the ethanol-induced GT enzyme for these substrates were 83 and 4.6 nmol/min/mg protein. The increases in catalytic efficiencies, apparent Vmax/Km for 1-naphthol and for morphine, for the ethanol-induced isozyme compared to the control isozyme activities were 2.0- and 2.4-fold. A polyclonal antibody raised in s...Continue Reading

References

May 1, 1985·Clinical Pharmacokinetics·E A LaneM Linnoila
Jul 1, 1986·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J M TredgerR Williams
May 1, 1984·Archives of Biochemistry and Biophysics·P I MackenzieI S Owens
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Jul 1, 1981·Archives of Biochemistry and Biophysics·R H Tukey, T R Tephly
Mar 1, 1983·Chemico-biological Interactions·T Y Aw, D P Jones

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Citations

Jan 1, 1992·Pharmacology & Therapeutics·F P Guengerich
Feb 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·C GuillemetteA Bélanger
May 1, 1991·The Journal of Pharmacy and Pharmacology·Z Ben-ZviJ Kaplanski
Jul 30, 2004·Alcohol·Sabine JurowichHerbert Käferstein
Apr 1, 1991·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S S NarayanG S Yost
Jan 1, 1994·Chemico-biological Interactions·H YinJ P Jones

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