PMID: 8961570Dec 1, 1996Paper

Purification and characterization of an exo-polygalacturonase from the tomato vascular wilt pathogen Fusarium oxysporum f.sp. lycopersici

FEMS Microbiology Letters
A Di Pietro, M I Roncero

Abstract

An exo-polygalacturonase (EC 3.2.1.15) was purified to apparent homogeneity from cultures of Fusarium oxysporum f.sp. lycopersici on synthetic medium supplemented with citrus pectin, using preparative isoelectric focusing. The enzyme, denominated PG2, had an apparent M(r) of 74000 Da upon SDS-PAGE. The pI of the main PG2 isoform was 4.5, and pH and temperature optima were 5.0 and 55 degrees C, respectively. PG2 hydrolyzed polygalacturonic acid in an exo-manner, as demonstrated by anaysis of degradation products. The enzyme was N-glycosylated. The N-terminal amino acid sequence, L-A-F-N-V-P-S-K-P-P, has no identify to other known polygalacturonases.

Citations

Jun 11, 2003·Molecular Plant-microbe Interactions : MPMI·Enid T González, Caitilyn Allen
Jun 2, 2009·Applied Biochemistry and Biotechnology·Andre Ricardo de Lima DamásioMaria de Lourdes Teixeira de Moraes Polizeli
Apr 8, 2000·Molecular Plant-microbe Interactions : MPMI·F I García-MaceiraM I Roncero
Apr 10, 2008·Environmental Microbiology·Daniela MinerdiAngelo Garibaldi
May 10, 2008·PLoS Pathogens·Petra M HoutermanMartijn Rep
Oct 22, 2015·The New Phytologist·Libera Lo PrestiRegine Kahmann
Mar 26, 2002·Microbiological Research·Maria Angélica dos Santos Cunha ChellegattiSuraia Said
Mar 10, 2004·Biotechnology and Bioengineering·I VerlentM E Hendrickx
Mar 17, 2009·Pest Management Science·David C Sands, Alice L Pilgeram
May 20, 2000·Biotechnology & Genetic Engineering Reviews·R A PradeA J Mort
Apr 10, 2015·International Journal of Molecular Sciences·Zhangyong Dong, Zhenzhong Wang
May 9, 1998·Applied and Environmental Microbiology· Di Pietro AM I G Roncero
Apr 18, 1998·Applied and Environmental Microbiology·J S Scott-CraigJ D Walton
Nov 28, 2009·Fungal Genetics and Biology : FG & B·M Asunción García-SánchezJosé María Díaz-Mínguez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.