PMID: 6980013May 25, 1982Paper

Purification and characterization of an N-acylphosphatidylserine from Rhodopseudomonas sphaeroides

Biochemistry
T J DonohueS Kaplan

Abstract

A new phospholipid that can account for up to 40% of the total cellular phospholipid of Rhodopseudomonas sphaeroides has been identified. Purification of the phospholipid was accomplished by column chromatography on silicic acid and diethylaminoethylcellulose followed by preparative thin-layer chromatography. A combination of spectroscopic and chemical techniques were used to identify the unknown phospholipid as an N-acylphosphatidylserine. Infrared spectroscopy revealed the presence of both ester and amide bonds in the phospholipid. Interpretation of the proton nuclear magnetic resonance spectrum of the new phospholipid indicated the presence of three acyl chains per phospholipid and in all other respects was compatible with the proposed structure of the molecule. Chemical studies confirmed the presence of a glycerylphosphorylserine moiety in the molecule and yielded three fatty acyl chains per hydrolyzed phospholipid. The fatty acid composition of the phospholipid was approximately 85% vaccenic acid, 9% stearic acid, 5% palmitic acid, and 1% palmitoleic acid, which is essentially identical with the fatty acid composition of whole cell phospholipid preparations from R. sphaeroides. Chemical synthesis of an N-acylphosphatidylse...Continue Reading

References

Jan 1, 1977·Methods in Enzymology· Landon
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C M GuptaH G Khorana
Jan 1, 1978·Annual Review of Biochemistry·J E Cronan
Mar 5, 1977·Journal of Molecular Biology·J E Rothman, E P Kennedy
Jun 1, 1978·Archives of Ophthalmology·R C Drews
Dec 29, 1967·Biochemical and Biophysical Research Communications·H E Carter, R C Gaver
Feb 18, 1972·Science·S J Singer, G L Nicolson
Jan 1, 1980·Annual Review of Biochemistry·R M Bell, R A Coleman
Jul 1, 1980·Journal of Bacteriology·D BaumgardnerS Kaplan
Jun 23, 1980·Biochimica Et Biophysica Acta·D E EppsH O Schmid
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

❮ Previous
Next ❯

Citations

Sep 16, 1991·Neuroscience Letters·Y LuS F Pang
Nov 12, 1993·Neuroscience Letters·C S PangS F Pang
Jan 3, 1994·Neuroscience Letters·C S PangS F Pang
Feb 8, 2014·Molecular Phylogenetics and Evolution·Kavita Kumari Kakarala, Kaiser Jamil
Apr 16, 1998·Journal of Photochemistry and Photobiology. B, Biology·J E RobertsJ F Wishart
Nov 23, 2007·Biochemistry·Ziqiang GuanChristian R H Raetz
Jul 23, 2011·BMC Systems Biology·Saheed ImamTimothy J Donohue
Aug 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Rachelle A S LemkeTimothy J Donohue
Mar 24, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ziqiang Guan
Jan 1, 1983·Journal of Cellular Biochemistry·S KaplanG S Yen
Mar 4, 2015·Journal of Bacteriology·Kimberly C LemmerTimothy J Donohue
Jan 27, 2017·Microbiology and Molecular Biology Reviews : MMBR·Harry A DaileyMartin J Warren
Feb 1, 1992·Protein Science : a Publication of the Protein Society·M G Eldridge, H A Dailey
Feb 1, 1983·Journal of Bacteriology·T B Campbell, D R Lueking
Aug 1, 1983·Journal of Bacteriology·T B Campbell, D R Lueking
Jan 1, 1990·Progress in Lipid Research·H H SchmidV Natarajan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.