PMID: 11929279Apr 4, 2002Paper

Purification and characterization of an antimicrobial chitinase extracellularly produced by Monascus purpureus CCRC31499 in a shrimp and crab shell powder medium

Journal of Agricultural and Food Chemistry
San-Lang WangWen-Teish Chang

Abstract

Monascus purpureus CCRC31499 produced an antimicrobial chitinase when it was grown in a medium containing shrimp and crab shell powder (SCSP) of marine wastes. An extracellular antimicrobial chitinase was purified from the culture supernatant to homology. The chitinase had a molecular weight of approximately 81,000 and a pI of 5.4. The optimal pH, optimum temperature, and pH stability of the chitinase were pH 7, 40 degrees C, and pH 6-8, respectively. The activity of the chitinase was activated by Fe(2+) and strongly inhibited by Hg(2+). The unique characteristics of the purified chitinase include high molecular weight, nearly neutral optimum pH, protease activity, and antimicrobial activity with bacteria and fungal phytopathogens. This is also the first report of isolation of a chitinase from a Monascus species.

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Citations

Aug 7, 2012·Applied Microbiology and Biotechnology·Masaaki YasudaMegumi Kuba-Miyara
Aug 28, 2012·Current Microbiology·Maria Swiontek Brzezinska, Urszula Jankiewicz
Jul 18, 2008·Applied Biochemistry and Biotechnology·Suraini Abd-AzizMohd Ali Hassan
Jun 1, 2010·Journal of Food Science and Technology·K Rathina Raj, N S Mahendrakar
Oct 1, 2008·International Journal of Biological Sciences·Benjaphorn PrapagdeeSkorn Mongkolsuk
Aug 31, 2013·Current Microbiology·Maria Swiontek BrzezinskaMaciej Walczak
Sep 4, 2007·Bioresource Technology·San-Lang WangYue-Horng Yen
Dec 24, 2015·Biotechnology Research International·Ivana A CavelloSebastián F Cavalitto
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Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·San-Lang WangAnh Dzung Nguyen
Jan 1, 2013·3 Biotech·Faouzi Ben Rebah, Nabil Miled
Aug 23, 2020·Applied Biochemistry and Biotechnology·Gincy M MathewAshok Pandey
Apr 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luyi JiangChu Chu
Mar 13, 2021·Archives of Microbiology·Neeraja P DholeRadhakrishna S Pandit
May 19, 2020·International Journal of Biological Macromolecules·Saquib MahmoodNeera Bhalla Sarin

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