Purification and characterization of an extracellular, uracil specific ribonuclease from a Bizionia species isolated from the marine environment of the Sundarbans

Microbiological Research
Barindra SanaJoydeep Mukherjee

Abstract

The first ribonuclease (RNase) from the Cytophaga-Flavobacterium-Bacteroides phylum, dominant in the marine environment, and also from the first Bizionia species isolated from the tropics was purified and characterized. Extracellular RNase production occurred when the culture medium contained 5-7% (w/v) NaCl. The 53.0 kDa enzyme was purified 29 folds with a recovery of 4% and specific activity of 630unit/mg protein. The pH and temperature optima are 6.5 and 35 degrees C, respectively and the enzyme retains more than half of its activity (relative to optimal assay conditions) after 1h pre-incubation separately with 5% (w/v) NaCl or from pH 5.0 to 8.5 or at 50 degrees C. Dithiothreitol and beta-mercaptoethanol do not inhibit whereas human placental RNase inhibitor protein halves the RNase activity. While Mg(2+), Ba(2+) and Ca(2+) enhanced the enzyme activity, Fe(2+), Cu(2+) and Hg(2+) inactivated it. This RNase degrades uracil containing nucleic acids only. Our isolate could be a novel renewable source of deoxyribonuclease (DNase)--free RNase enzyme.

References

Jun 1, 1986·Journal of Biochemical and Biophysical Methods·K Isobe, S Uchiyama
Oct 1, 1996·Applied and Environmental Microbiology·E W AlmL Raskin
Jun 17, 1999·FEMS Microbiology Reviews·A W Nicholson
Jan 8, 2000·The Journal of Biological Chemistry·C CallahanM P Deutscher
Dec 20, 2002·Nucleic Acids Research·Ciarán Condon, Harald Putzer
Jan 5, 2005·Biochemical and Biophysical Research Communications·Vladimir R Kaberdin, Thierry Bizebard
Mar 19, 2005·International Journal of Systematic and Evolutionary Microbiology·Hana YiJongsik Chun
Mar 1, 1993·World Journal of Microbiology & Biotechnology·P S ApteV Shankar

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Citations

Jan 25, 2011·Nucleosides, Nucleotides & Nucleic Acids·Periasamy Ashok Kumar, Vijayaraghavan Kannan
Jun 11, 2011·Se pu = Chinese journal of chromatography·Xiaoming Yang, Xindu Geng
Jan 22, 2017·International Journal of Biological Macromolecules·Gupta ShrutiKanwar Shamsher Singh
Jul 20, 2019·Preparative Biochemistry & Biotechnology·Rakesh Kumar, Shamsher Singh Kanwar
Nov 3, 2020·International Journal of Biological Macromolecules·Rakesh KumarShamsher Singh Kanwar

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