Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3

Journal of Agricultural and Food Chemistry
Marit ReitsmaHarry Wichers

Abstract

In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.

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Citations

Sep 3, 2016·Clinical Reviews in Allergy & Immunology·Cíntia MendesIsabel Mafra
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Dec 15, 2016·Journal of Agricultural and Food Chemistry·Christopher P MattisonRichard L Wasserman
Oct 29, 2018·International Archives of Allergy and Immunology·Shanna Bastiaan-NetHarry J Wichers

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