PMID: 6982067Aug 17, 1982Paper

Purification and characterization of androgen receptor from steer seminal vesicle

Biochemistry
C H ChangD J Tindall

Abstract

The androgen receptor has been purified from steer seminal vesicle cytosol by a combination of differential DNA-Sepharose 4B chromatography and testosterone 17 beta-hemisuccinyl-3,3'-diaminodipropylamine-Sepharose 4B affinity chromatography. The procedure produced about 3 micrograms of receptor protein from 35 g of steer seminal vesicle, with a yield of 48%. The receptor protein, as a complex with unlabeled testosterone, was purified approximately 540000-fold. A single band, migrating at 60000 daltons, was observed following electrophoresis on a polyacrylamide gel containing sodium dodecyl sulfate (NaDoDSO4). This was confirmed by affinity labeling of the partially purified receptor with both 17-hydroxy-17 alpha-[3H]methyl-4,9,11-estratrien-3-one and 17 beta-hydroxy-[1,2,4,5,6,7,16,17-3H8)-5 alpha-androstan-3-one 17-(2-bromoacetate), which showed a peak of radioactivity migrating at 60000 daltons by NaDoDSO4 gel electrophoresis. The receptor had an estimated Stokes radius of 35 A and a sedimentation coefficient of 3.8 S in the presence of 0.3 M NaCl. The calculated molecular weight and frictional ratio for the androgen binding activity were 57000 and 1.42, respectively. Chromatofocusing of the purified receptor protein revealed...Continue Reading

References

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Citations

Oct 1, 1984·Journal of Steroid Biochemistry·S L CarrollD J Tindall
May 1, 1987·Journal of Steroid Biochemistry·J O Macaulay, G L Warne
May 1, 1988·Journal of Steroid Biochemistry·J O MacaulayZ S Krozowski
Apr 1, 1989·Journal of Steroid Biochemistry·J O MacaulayG L Warne
Dec 1, 1989·Journal of Steroid Biochemistry·D L Ellis, B J Danzo
Jan 1, 1984·Pharmacology & Therapeutics·D J TindallG R Cunningham
Jun 1, 1986·Molecular and Cellular Endocrinology·H Gronemeyer, M V Govindan
Dec 1, 1987·Molecular and Cellular Endocrinology·D StamatiadisP Mauvais-Jarvis
Jan 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·D StamatiadisP Mauvais-Jarvis
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P Ghosh-DastidarC F Fox
Aug 12, 1983·Biochemical and Biophysical Research Communications·E MulderA O Brinkmann
Jan 1, 1984·Annals of the New York Academy of Sciences·D J TindallD R Rowley
Jan 1, 1986·The Prostate·L R MurthyD J Tindall
Jan 16, 1985·Biochemical and Biophysical Research Communications·A O BrinkmannH J van der Molen
Mar 28, 1986·Biochemical and Biophysical Research Communications·B Ofenloch-HähnleK Eisele
Jun 1, 1992·The Journal of Investigative Dermatology·M E Sawaya
Sep 15, 1988·Biochemical and Biophysical Research Communications·K WrogemannE Rosenmann

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