Purification and characterization of avian beta-defensin 11, an antimicrobial peptide of the hen egg.

Antimicrobial Agents and Chemotherapy
Virginie Hervé-GrépinetYves Nys

Abstract

Natural antimicrobial peptides are present in different compartments (eggshell, egg white, and vitelline membranes) of the hen egg and are expected to be involved in the protection of the embryo during its development and to contribute to the production of pathogen-free eggs. In the present study, we used vitelline membranes from hen (Gallus gallus) eggs as a source of avian β-defensin 11 (AvBD11). A purification scheme using affinity chromatography and reverse-phase chromatography was developed. Purified AvBD11 was analyzed by a combination of mass spectrometry approaches to characterize its primary sequence and structure. A monoisotopic molecular species at [M + H](+) of 9,271.56 Da was obtained, and its N- and C-terminal sequences were determined. We also examined posttranslational modifications and identified the presence of 6 internal disulfide bonds. AvBD11 was found to exhibit antimicrobial activity toward both Gram-positive and Gram-negative bacteria.

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Citations

Feb 6, 2013·Veterinary Research Communications·Maria AnastasiadouGeorgios Michailidis
Apr 26, 2013·The Journal of Biological Chemistry·Sophie Réhault-GodbertYves Nys
May 14, 2014·Journal of Agricultural and Food Chemistry·Kenji MaehashiToshihiro Watanabe
Jun 12, 2014·Pharmaceuticals·Monique L van Hoek
Jan 15, 2015·Journal of Proteomics·Megan Rose-MartelMaxwell T Hincke
Nov 26, 2015·Cytokine & Growth Factor Reviews·Ehab H MattarElrashdy M Redwan
Dec 16, 2011·Immunological Reviews·Robert I Lehrer, Wuyuan Lu
Mar 22, 2014·Journal of the Science of Food and Agriculture·Zhipeng YuJingbo Liu
Aug 3, 2014·Journal of Proteomics·Valérie LabasElisabeth Blesbois
Sep 17, 2015·BMC Evolutionary Biology·Yuanyuan ChengDavid W Burt
Oct 11, 2013·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Hayfaa A Al-Shammary
May 7, 2013·Developmental and Comparative Immunology·Tryntsje CuperusHenk P Haagsman
Jan 15, 2017·Journal of Proteomics·Tamer A E AhmedMaxwell T Hincke
Mar 26, 2016·Biochemical and Biophysical Research Communications·Iara Fernanda RochaJosé Luis da Conceição Silva
Jan 21, 2014·The Journal of Biological Chemistry·Virginie HervéCéline Landon
Feb 28, 2019·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Louis Alex JulienSimon Colin Andrews
Feb 16, 2018·Molecular & Cellular Proteomics : MCP·Mylène Da SilvaSophie Réhault-Godbert
Dec 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Nicolas GuyotCéline Landon
Mar 15, 2019·Molecular & Cellular Proteomics : MCP·Mylène Da SilvaSophie Réhault-Godbert
Mar 8, 2018·Journal of Agricultural and Food Chemistry·Simona Arena, Andrea Scaloni

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