PMID: 2179198Jan 1, 1990

Purification and characterization of cellulolytic enzymes produced by Aspergillus nidulans

The Journal of Applied Bacteriology
P S BaggaS Sharma

Abstract

Three exo-glucanases, two endo-glucanases and two beta-glucosidases were separated and purified from the culture medium of Aspergillus nidulans. The optimal assay conditions for all forms of cellulase components ranged from pH 5.0 to 6.0 and 50 degrees C and 65 degrees C for exo-glucanases and endo-glucanases but 35 degrees C and 65 degrees C for beta-glucosidases. A close relation of enzyme stability to their optimal pH range was observed. All the cellulase components were stable for 10 min at 40-50 degrees C. Exo-II and Exo-III (Km, 38.46 and 37.71 mg/ml) had greater affinity for the substrate than Exo-I (Km, 50.00 mg/ml). The Km values of Endo-I and Endo-II (5.0 and 4.0 mg/ml) and their maximum reaction velocities (Vmax, 12.0 and 10.0 IU/mg protein) were comparable. beta-Glucosidases exhibited Km values of 0.24 and 0.12 mmol and Vmax values of 8.00 and 0.67 IU/mg protein. The molecular weights recorded for various enzyme forms were: Exo-I, 29,000; Exo-II, 72,500; Exo-III, 138,000; Endo-I, 25,000; Endo-II, 32,500; beta-Gluco-I, 14,000 and beta-Gluco-II, 26,000. Exo- and endo-glucanases were found to require some metal ions as co-factors for their catalytic activities whereas beta-glucosidases did not. Hg2+ inhibited the activ...Continue Reading

References

Jan 1, 1979·Folia Microbiologica·J K Gupta, Y P Gupta
Jan 1, 1981·International Journal of Immunopharmacology·D D Wood, M J Staruch
Mar 1, 1982·Biotechnology and Bioengineering·S K Garg, S Neelakantan
Jan 1, 1984·Biotechnology and Bioengineering·M VaidyaM Rao

Citations

Jan 1, 1996·Journal of Basic Microbiology·S SinghA Kaur
Jan 1, 1997·Journal of Basic Microbiology·R K Harchand, S Singh
Feb 1, 1990·Biochemical Genetics·S SharmaP S Bagga
May 25, 2011·Chemical Communications : Chem Comm·Yasuhito SuganoEiichi Tamiya
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·R P de Vries, J Visser
Jul 28, 2012·Biotechnology for Biofuels·Sayali SaykhedkarAndrew J Mort
Jun 19, 2004·Peptides·T B Ng
May 1, 1997·The Journal of Eukaryotic Microbiology·R L Jacobson, Y Schlein

Related Concepts

Aspergillus nidulans
Endoglucanase Y
Glucosidase
Hydrogen-Ion Concentration
Soil Microbiology

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