PMID: 9443067Jan 27, 1998Paper

Purification and characterization of cytochrome P450 reductase from liver microsomes of feral leaping mullet (Liza saliens)

Journal of Biochemical and Molecular Toxicology
Alaattin Sen, Emel Arinç

Abstract

NADPH-cytochrome P450 reductase was purified to electrophoretic homogeneity from detergent-solubilized liver microsomes from the leaping mullet (Liza saliens). The purified reductase was characterized with respect to spectral, electrophoretic, and biocatalytic properties. In addition, effects of pH, ionic strength, and the substrate concentration on the NADPH-dependent cytochrome c reductase activity of the purified fish liver cytochrome P450 reductase were studied. Cytochrome P450 reductase was purified 438-fold with a yield of 17.5% with respect to the initial amount present in the fish liver microsomes. The specific activity of the enzyme was found to be 52.6 mumol cytochrome c reduced per minute per mg protein. The monomer molecular weight of the purified enzyme was calculated to be 77,000 +/- 1000 when electrophoresed on polyacrylamide gels under the denaturing conditions in the presence of SDS. The absorption spectrum of fish reductase showed two peaks at 378 and 455 nm. NADPH-dependent cytochrome c reductase activity of the purified Liza saliens liver cytochrome P450 reductase was found to be maximal when pH was between 7.4 and 7.8. The apparent K(m) of the purified enzyme was found to be 7.69 microM for cytochrome c whe...Continue Reading

Citations

Oct 26, 2000·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·A Sen, E Arinç
Feb 11, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·A Sen, E Arinç
Mar 10, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·E ArinçE Su
Feb 14, 2009·Marine Environmental Research·Azra BozcaarmutluEmel Arinç
Nov 23, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Azra Bozcaarmutlu, Emel Arinç
Aug 30, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Haydar Celik, Emel Arinç
Dec 23, 2016·Archives of Toxicology·Q ShiR W Godschalk
Dec 14, 2002·Journal of Biochemical and Molecular Toxicology·Emel ArinçHaydar Lelik
Jun 20, 2003·Journal of Biochemical and Molecular Toxicology·Emel Arinç, Azra Bozcaarmutlu

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