Purification and characterization of five variants of phospholipase A2 and complete primary structure of the main phospholipase A2 variant in Heloderma suspectum (Gila monster) venom.

European Journal of Biochemistry
F GomezJ Christophe

Abstract

1. Five increasingly anionic variants (Pa1-Pa5) of Ca2+-dependent phospholipase A2 were purified to homogeneity from the venom of the lizard Heloderma suspectum (Gila monster). The purification procedure was based on semi-preparative reverse-phase HPLC followed by anion-exchange HPLC and analytical reverse-phase HPLC. 2. Their Mr were 17,000-18,000, as deduced by SDS/PAGE. Specific activities tested by the capacity to hydrolyze phosphatidylcholines at pH 8.5 decreased as follows: Pa3 greater than Pa5 greater than Pa4 greater than Pa1 greater than Pa2. These activities showed the same optimum pH (9.0), were mainly of the phospholipase A2 type and were lost upon p-bromophenacyl bromide treatment. 3. All five phospholipases efficiently stimulated amylase release from dispersed rat pancreatic acini at pH 7.4, their potency decreasing as follows: Pa2 greater than Pa1 approximately equal to Pa4 greater than Pa3 approximately equal to Pa5. No deleterious effect was apparent based on the lack of lactate dehydrogenase release. 4. The five variants, Pa1-Pa5, differed significantly in amino acid composition and this, together with distinct antigenic properties of Pa2 and Pa5, establishes the subheterogeneity of this new type of phospholip...Continue Reading

References

Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Sep 5, 1978·Journal of Molecular Biology·B W DijkstraP J Vandermaelen
Dec 16, 1975·Biochemistry·A J Slotboom, G H de Haas
Nov 5, 1987·Biochimica Et Biophysica Acta·I H TsaiT Chang
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Oct 2, 1974·European Journal of Biochemistry·R A ShipoliniC A Vernon
Mar 26, 1974·Biochemistry·J J VolwerkG H de Haas
Dec 7, 1984·Journal of Chromatography·B A BidlingmeyerT L Tarvin
Feb 15, 1984·European Journal of Biochemistry·J H van EijkG H de Haas
Sep 2, 1983·European Journal of Pharmacology·J P DehayeJ Christophe
Jan 1, 1981·Reviews of Physiology, Biochemistry and Pharmacology·H M VerheijG H de Haas
Mar 25, 1981·Journal of Molecular Biology·B W DijkstraJ Drenth

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Citations

Sep 4, 2015·Toxicon : Official Journal of the International Society on Toxinology·Imen JridiLamia Borchani
Jul 9, 2013·Toxicon : Official Journal of the International Society on Toxinology·Hanen LouatiYoussef Gargouri
Nov 13, 2012·Toxicon : Official Journal of the International Society on Toxinology·Paroonkorn IncamnoiSakda Daduang
Jan 21, 2015·Journal of Proteomics·Kristian W SanggaardJan J Enghild
Jun 8, 1995·Nature·A LotanE Zlotkin
Jan 24, 1998·The Journal of Peptide Research : Official Journal of the American Peptide Society·G Datta, A T Tu
Mar 28, 1991·European Journal of Biochemistry·A VandermeersJ Christophe
Nov 18, 2000·The Journal of Biological Chemistry·S R DowdJ L Patton-Vogt
Dec 31, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Md Sakib HossenMd Ibrahim Khalil
Nov 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·M NakaiM Ohno
Jun 1, 2021·F1000Research·Yuliana EmilianiDilia Aparicio

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