Purification and characterization of ginsenoside-beta-glucosidase from ginseng

Chemical & Pharmaceutical Bulletin
C ZhangFengxie Jin

Abstract

The ginsenoside-beta-glucosidase that hydrolyzes the beta-(1-->2)-glucoside of the ginsenoside Rg3 sugar moiety to ginsenoside Rh2 was isolated from the ginseng root, and the enzyme was purified and characterized. The enzyme was purified to one spot in SDS polyacrylamide gel electrophoresis, and its molecular weight was about 59 kDa. The optimum temperature of the ginsenoside-beta-glucosidase was 60 degrees C, and the optimum pH was 5.0. Ca2+ ion had positive effect on ginsenoside-beta-glucosidase, while Cu2+ had negative effect on it. The ginsenoside-beta-glucosidase may be a special beta-glucosidase that is different from the original exocellulase such as beta-glucosidase (EC 3.2.1.21).

Citations

Jul 10, 2012·Chinese Journal of Integrative Medicine·Si-Dao ZhengDe-Lin Wu
Feb 19, 2002·Chemical & Pharmaceutical Bulletin·Hongshan YuFengxie Jin
Sep 19, 2015·Critical Reviews in Biotechnology·Kyung-Chul Shin, Deok-Kun Oh
Jan 31, 2004·Phytochemistry·David G Popovich, David D Kitts
Aug 5, 2005·Chemical & Pharmaceutical Bulletin·Srguleng QianFengxie Jin
Aug 9, 2006·Acta Biochimica Et Biophysica Sinica·Doungkamol BoonclarmJisnuson Svasti

Related Concepts

Ginsenoside-beta-glucosidase
Emulsion beta-D-Glucosidase
Chromatography, DEAE-Cellulose
Thin Layer Chromatography
Panax ginseng
Hydrolysis
Plant Extracts
Plant Proteins
Saponin
Ginsenosides

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