PMID: 6981429Jul 26, 1982Paper

Purification and characterization of human C1-esterase inhibitor

Biochimica Et Biophysica Acta
T Nilsson, B Wiman

Abstract

A new purification method for C1-esterase inhibitor is described, which is essentially a three-step procedure: precipitation with poly(ethylene glycol), chromatography on DEAE-cellulose and hydrophobic interaction chromatography on hexyl-Sepharose. The final product is a single-chain glycoprotein with a molecular weight of about 100 000 and NH2-terminal asparagine. The molecule is fully active as judged by complex formation with C1s. Two of its three disulphide bridges can be easily reduced and S-carboxymethylated under non-denaturing conditions without loss of activity. However, at high dithioerythritol concentration the third disulphide bridge is also cleaved and accompanied by loss of the activity, indicating that this disulphide bridge is involved in maintaining the conformation around the reactive site in the inhibitor.

References

Jun 1, 1977·European Journal of Biochemistry·B Wiman
Sep 1, 1976·The Biochemical Journal·I GigliR B Sim
Jan 1, 1976·Methods in Enzymology·P C Harpel
Dec 1, 1970·European Journal of Biochemistry·H HauptH G Schwick

❮ Previous
Next ❯

Citations

Sep 1, 1987·Clinical Rheumatology·T K Nilsson
Mar 14, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·B Wiman, T Nilsson
Jun 2, 1989·Journal of Immunological Methods·Y PilatteL F Fries
Aug 15, 1998·Vox Sanguinis·S Meri, H Jarva
Nov 21, 2002·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·E W BrownP A Patston
Jan 1, 1983·European Journal of Biochemistry·T Nilsson, B Wiman
Mar 1, 1983·Scandinavian Journal of Haematology·T NilssonB Cedergren
Sep 27, 2000·Biochimica Et Biophysica Acta·I Simonovic, P A Patston
Jul 1, 1986·Biochemistry·M LennickK C Ingham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.