PMID: 6270633Jan 1, 1981

Purification and characterization of human converting enzyme (kininase II)

Peptides
T A StewartE G Erdös

Abstract

We purified peptidyl-dipeptidase (converting enzyme, EC 3.4.15.1) to homogeneity from the membrane fraction of human lung and for comparison, from human and hog kidney. The membrane-bound lung enzyme was purified 1800-fold with 19% yield, and the kidney enzyme 640-fold with 10% yield. The specific activities with Bz-Gly-His-Leu were 81 mumol/min/mg for the lung and 65 for the kidney enzyme. The lung enzyme was homogeneous in gel electrophoresis with Mr = 155,000 and Sw,20 = 8.0 in ultracentrifugation. Antibodies elicited against lung or kidney enzyme cross-reacted with enzyme from other organ, but not with the hog enzyme. In isoelectric focusing both human enzymes had a major form with a pI of 5.2. The lung preparation also contained more acidic forms (pI = 4--5), which were eliminated by treatment with neuraminidase. Lung and kidney converting enzyme hydrolyzed bradykinin, angiotensin I, and enkephalins and had similar kinetic constants. Bradykinin was the best substrate, as indicated by its kcat/Km, but Met5-enkephalin had the highest turnover number. The hydrolysis of Bz-Gly-His-Leu was inhibited by captopril (SQ 14225) competitively, and by Keto-ACE, a non-peptide derivative of Bz-Phe-Gly-Pro, non-competitively.

References

Nov 1, 1977·The Biochemical Journal·J W RyanM L Carlton
Jun 1, 1979·Analytical Biochemistry·B HolmquistJ F Riordan
Apr 20, 1968·Lancet·G Dale, A L Latner

Citations

Dec 1, 1993·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·F F JungJ R Ingelfinger
Apr 1, 1989·Archives of Biochemistry and Biophysics·Y DasarathyJ J Lanzillo
Jan 1, 1989·Life Sciences·J R Schullek, I B Wilson
Jul 16, 1986·Biochimica Et Biophysica Acta·H H Bausback, P E Ward
Jan 20, 1987·Biochimica Et Biophysica Acta·I Y SakharovE A Dukhanina
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry· Sakharov IYu
May 29, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T KawamuraT Muramatsu
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A Skidgel, E G Erdös
Jan 1, 1987·Clinical and Experimental Hypertension. Part A, Theory and Practice·T Kokubu, Y Takada
Aug 10, 1987·FEBS Letters·D ChanselR Ardaillou
Mar 1, 1993·Journal of Neurochemistry·J Brownlees, C H Williams
Jan 27, 1983·Biochemical and Biophysical Research Communications·L B HershE G Erdös
Oct 1, 1986·Metabolism: Clinical and Experimental·R C SmallridgeC Eil
Dec 1, 1983·Archives of Biochemistry and Biophysics·J J LanzilloB L Fanburg
Nov 1, 1985·Placenta·H TairaY Tomoda
Jan 1, 1982·Annals of the New York Academy of Sciences·N MarksL Sachs
Oct 1, 1987·Analytical Biochemistry· Sakharov IYuN V Sukhova
Jan 1, 1983·Clinical and Experimental Hypertension. Part A, Theory and Practice·E G Erdös, J T Gafford
Jun 27, 1989·Biochemistry·M R Ehlers, J F Riordan
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·A KuoppalaJ O Kokkonen

Related Concepts

Metazoa
Plasma Membrane
Immunodiffusion Measurement
Kidney
Peptidyl-Dipeptidase A
Lung
Tissue Specificity
Substrate Specificity
Family suidae

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