PMID: 6270633Jan 1, 1981

Purification and characterization of human converting enzyme (kininase II)

T A StewartE G Erdös


We purified peptidyl-dipeptidase (converting enzyme, EC to homogeneity from the membrane fraction of human lung and for comparison, from human and hog kidney. The membrane-bound lung enzyme was purified 1800-fold with 19% yield, and the kidney enzyme 640-fold with 10% yield. The specific activities with Bz-Gly-His-Leu were 81 mumol/min/mg for the lung and 65 for the kidney enzyme. The lung enzyme was homogeneous in gel electrophoresis with Mr = 155,000 and Sw,20 = 8.0 in ultracentrifugation. Antibodies elicited against lung or kidney enzyme cross-reacted with enzyme from other organ, but not with the hog enzyme. In isoelectric focusing both human enzymes had a major form with a pI of 5.2. The lung preparation also contained more acidic forms (pI = 4--5), which were eliminated by treatment with neuraminidase. Lung and kidney converting enzyme hydrolyzed bradykinin, angiotensin I, and enkephalins and had similar kinetic constants. Bradykinin was the best substrate, as indicated by its kcat/Km, but Met5-enkephalin had the highest turnover number. The hydrolysis of Bz-Gly-His-Leu was inhibited by captopril (SQ 14225) competitively, and by Keto-ACE, a non-peptide derivative of Bz-Phe-Gly-Pro, non-competitively.


Nov 1, 1977·The Biochemical Journal·J W RyanM L Carlton
Jun 1, 1979·Analytical Biochemistry·B HolmquistJ F Riordan
Apr 20, 1968·Lancet·G Dale, A L Latner


Dec 1, 1993·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·F F JungJ R Ingelfinger
Apr 1, 1989·Archives of Biochemistry and Biophysics·Y DasarathyJ J Lanzillo
Jan 1, 1989·Life Sciences·J R Schullek, I B Wilson
Jul 16, 1986·Biochimica Et Biophysica Acta·H H Bausback, P E Ward
Jan 20, 1987·Biochimica Et Biophysica Acta·I Y SakharovE A Dukhanina
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry· Sakharov IYu
May 29, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T KawamuraT Muramatsu
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A Skidgel, E G Erdös
Jan 1, 1987·Clinical and Experimental Hypertension. Part A, Theory and Practice·T Kokubu, Y Takada
Aug 10, 1987·FEBS Letters·D ChanselR Ardaillou
Mar 1, 1993·Journal of Neurochemistry·J Brownlees, C H Williams
Jan 27, 1983·Biochemical and Biophysical Research Communications·L B HershE G Erdös
Oct 1, 1986·Metabolism: Clinical and Experimental·R C SmallridgeC Eil
Dec 1, 1983·Archives of Biochemistry and Biophysics·J J LanzilloB L Fanburg
Nov 1, 1985·Placenta·H TairaY Tomoda
Jan 1, 1982·Annals of the New York Academy of Sciences·N MarksL Sachs
Oct 1, 1987·Analytical Biochemistry· Sakharov IYuN V Sukhova
Jan 1, 1983·Clinical and Experimental Hypertension. Part A, Theory and Practice·E G Erdös, J T Gafford
Jun 27, 1989·Biochemistry·M R Ehlers, J F Riordan
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·A KuoppalaJ O Kokkonen

Related Concepts

Plasma Membrane
Immunodiffusion Measurement
Peptidyl-Dipeptidase A
Tissue Specificity
Substrate Specificity
Family suidae

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.