Purification and characterization of lymphocytic clonal growth factor (LCGF) derived from human-human hybridoma SH-76 cells.

Cytotechnology
M MiyataH Omura

Abstract

Human-human hybridoma SH-76 cells were found to produce a factor that supported the growth of lymphocytic cells at low densities. The factor was purified from serum-free conditioned medium of the hybridoma cells by a successive application of ammonium sulfate precipitation, DEAE-Toyopearl, TSK G3000 SW and DEAE-5PW column chromatograph. The purified factor was a 72K single protein. The factor showed marked growth stimulating effect on lymphocytic cell lines, but had no effect on the growth of human adhesive cancer cell lines. Thus, the factor is a lymphocytic clonal growth factor (LCGF), as found previously in human plasma (Miyata, 1988). The LCGF of SH-76 cells could be produced in growth factor-free RPMI medium and purified easily from the conditioned medium. The factor is inactivated by heating at over 80°C, but is much more stable than the LCGF in human plasma.

References

Feb 1, 1986·Cell Biology International Reports·H OhashiH Omura
Jan 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S HashizumeH Omura
Oct 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H MurakamiH Omura
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H MurakamiT Kano-Sueoka
Feb 5, 1988·Biotechnology and Bioengineering·Y TakazawaH Omura

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