Purification and characterization of lysozyme from plasma of the eastern oyster (Crassostrea virginica)

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Qinggang XueJerome F La Peyre

Abstract

Lysozyme was purified from the plasma of eastern oysters (Crassostrea virginica) using a combination of ion exchange and gel filtration chromatographies. The molecular mass of purified lysozyme was estimated at 18.4 kDa by SDS-PAGE, and its isoelectric point was greater than 10. Mass spectrometric analysis of the purified enzyme revealed a high-sequence homology with i-type lysozymes. No similarity was found however between the N-terminal sequence of oyster plasma lysozyme and N-terminal sequences of other i-type lysozymes, suggesting that the N-terminal sequences of the i-type lysozymes may vary to a greater extent between species than reported in earlier studies. The optimal ionic strength, pH, cation concentrations, sea salt concentrations, and temperature for activity of the purified lysozyme were determined, as well as its temperature and pH stability. Purified oyster plasma lysozyme inhibited the growth of Gram-positive bacteria (e.g., Lactococcus garvieae, Enterococcus sp.) and Gram-negative bacteria (e.g., Escherichia coli, Vibrio vulnificus). This is a first report of a lysozyme purified from an oyster species and from the plasma of a bivalve mollusc.

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Citations

Apr 24, 2010·Journal of Biosciences·Lien Callewaert, Chris W Michiels
Jul 23, 2013·Journal of Invertebrate Pathology·Philippe SoudantAswani Volety
Mar 20, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yuki OkadaKeisuke G Takahashi
Jun 27, 2014·Developmental and Comparative Immunology·Na XuShicui Zhang
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Jun 3, 2014·Fish & Shellfish Immunology·Yunhai Guo, Hongxuan He
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Dec 3, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yi TianJian Song
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May 15, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Qinggang XueJerome La Peyre
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Sep 8, 2010·Fish & Shellfish Immunology·Hong-Wei ZhangJin-Xing Wang

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