Purification and characterization of mouse soluble receptor for advanced glycation end products (sRAGE).

The Journal of Biological Chemistry
Lana E HanfordTim D Oury

Abstract

The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily of cell surface proteins that has been implicated as a progression factor in a number of pathologic conditions from chronic inflammation to cancer to Alzheimer's disease. In such conditions, RAGE acts to facilitate pathogenic processes. Its secreted isoform, soluble RAGE or sRAGE, has the ability to prevent RAGE signaling by acting as a decoy. sRAGE has been used successfully in animal models of a range of diseases to antagonize RAGE-mediated pathologic processes. In humans, sRAGE results from alternative splicing of RAGE mRNA. This study was aimed to determine whether the same holds true for mouse sRAGE and, in addition, to biochemically characterize mouse sRAGE. The biochemical characteristics examined include glycosylation and disulfide patterns. In addition, sRAGE was found to bind heparin, which may mediate its distribution in the extracellular matrix and cell surfaces of tissues. Finally, our data indicated that sRAGE in the mouse is likely produced by carboxyl-terminal truncation, in contrast to the alternative splicing mechanism reported in humans.

References

Aug 31, 2000·Biochemical and Biophysical Research Communications·C L FattmanT D Oury
Dec 8, 2000·Biochimica Et Biophysica Acta·A M SchmidtD M Stern
Nov 18, 2003·The Journal of Biological Chemistry·Thiruvengadam ArumugamCraig D Logsdon
Apr 21, 2004·Annals of Plastic Surgery·Kelley Wear-MaggittiArnold Breitbart

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Citations

Sep 1, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Angelika BierhausPeter P Nawroth
Jul 11, 2012·Intensive Care Medicine·Weidun Alan GuoKrishnan Raghavendran
Apr 2, 2005·Nature Reviews. Immunology·Michael T Lotze, Kevin J Tracey
Nov 26, 2013·Genome Biology and Evolution·Raquel López-DíezBegoña Aguado
Jan 24, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anastasia Z KaleaBarry I Hudson
Apr 7, 2007·Critical Care Medicine·Helmut WittkowskiDirk Foell
Feb 23, 2008·Clinical and Experimental Pharmacology & Physiology·Karin Jandeleit-DahmAino Soro-Paavonen
Feb 8, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anja H HergrueterCaroline A Owen
Jan 25, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Naoko YamakawaKoshi Makita
May 5, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiao SuMichael A Matthay
Feb 4, 2006·American Journal of Respiratory and Critical Care Medicine·Tokujiro UchidaMichael A Matthay
Apr 19, 2008·American Journal of Respiratory Cell and Molecular Biology·Markus A QueisserKlaus T Preissner
Oct 8, 2011·American Journal of Respiratory Cell and Molecular Biology·Damien J LaparVictor E Laubach
Mar 19, 2009·Journal of Translational Medicine·Louis J SparveroMichael T Lotze
Aug 30, 2011·Critical Care : the Official Journal of the Critical Care Forum·Yaseen M ArabiAbderrezak Bouchama
Jan 30, 2008·Critical Care : the Official Journal of the Critical Care Forum·Christian BoppMarkus A Weigand
May 21, 2009·Journal of Leukocyte Biology·Shaival H DavéScott E Plevy
Apr 24, 2007·Environmental Health Perspectives·R Clark LantzJefferey L Burgess
Jul 20, 2011·Journal of Perinatal Medicine·Jae-Hyun KwonYong-Won Park
Oct 13, 2007·Molecular Medicine·Hidenori KoyamaYoshiki Nishizawa
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Li LinEdward G Lakatta
Oct 9, 2013·International Journal of Inflammation·Yaw Kuang ChuahNorshariza Nordin
May 16, 2013·Cellular and Molecular Life Sciences : CMLS·Koen Van CrombruggenClaus Bachert
Feb 11, 2010·Journal of Biomedicine & Biotechnology·Stephen T Buckley, Carsten Ehrhardt
Aug 27, 2013·Expert Review of Clinical Immunology·Shui-Lian YuLai-Shan Tam
Jun 30, 2006·Expert Opinion on Biological Therapy·Christian A PetrulioHoward L Kaufman
Mar 17, 2016·Disease Markers·Raiko BlondonnetMatthieu Jabaudon

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