Purification and characterization of natural Ara h 8, the Bet v 1 homologous allergen from peanut, provides a novel isoform

Biological Chemistry
Susanne RieckenWolf-Meinhard Becker

Abstract

The peanut allergen Ara h 8 is an important allergen for birch pollen allergic patients because of the cross-reactivity to the homologous Bet v 1. As the existence of Ara h 8 has been shown at the cDNA level so far (AY328088) and the allergen has indirectly been detected as natural protein, it was the aim of our study to identify natural Ara h 8 in peanut extract and to develop a purification strategy. This was achieved using a unique combination of purification steps, including optimized extraction conditions, size exclusion and ion exchange chromatography and treatment of the interfering contaminants with iodoacetic acid. A characterization of the protein by microsequencing showed discrepancies to the deduced amino acid sequence of AY328088. For this reason, we cloned and expressed a new Ara h 8 isoform from cDNA (EU046325). This IgE-reactive protein corresponds to the results of microsequencing, ESI-FTICR-MS and trypsin fingerprinting analysis of the authentic and purified nAra h 8. Apart from the ultimate use of recombinant allergens for diagnostic procedures, there is also a scientific need for the natural counterpart, as it represents an excellent reference point by which to compare protein characteristics and to standard...Continue Reading

References

Jan 1, 1992·International Archives of Allergy and Immunology·A PetersenM Schlaak
Aug 1, 1983·Analytical Biochemistry·K Hancock, V C Tsang
Apr 1, 1995·The Journal of Allergy and Clinical Immunology·S L HefleR K Bush
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Mar 1, 1997·Nucleic Acids Research·A J McCullough, M A Schuler
Sep 4, 1999·International Archives of Allergy and Immunology·T Kleber-JankeW M Becker
Mar 31, 2000·Trends in Plant Science·Z J LorkovićW Filipowicz
Jul 11, 2000·The Journal of Allergy and Clinical Immunology·H Breiteneder, C Ebner
Feb 15, 2001·The Journal of Allergy and Clinical Immunology·H S SkolnickR A Wood
Nov 6, 2002·The Journal of Allergy and Clinical Immunology·Jörg Kleine-TebbeStefan Vieths
Nov 5, 2003·Molecular Immunology·Olga M ViquezHortense W Dodo
Nov 6, 2003·International Archives of Allergy and Immunology·Jaap H AkkerdaasRonald van Ree
Dec 24, 2003·The Plant Journal : for Cell and Molecular Biology·Chang-Jin ParkKyung-Hee Paek
Oct 16, 2004·Molecular Immunology·Olga M ViquezHortense W Dodo
Nov 16, 2004·Critical Reviews in Food Science and Nutrition·E N Clare MillsPeter R Shewry
Aug 27, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D MittagB K Ballmer-Weber
Oct 13, 2005·International Archives of Allergy and Immunology·Fabrizio GuarneriSalvatore Benvenga
Jul 4, 2006·The Journal of Allergy and Clinical Immunology·Catherine AstierGisèle Kanny

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Citations

May 4, 2013·Critical Reviews in Food Science and Nutrition·Jorge SáizCarmen García-Ruiz
Feb 21, 2014·Current Allergy and Asthma Reports·Merima Bublin, Heimo Breiteneder
Apr 4, 2014·TheScientificWorldJournal·Mau SinhaTej P Singh
Sep 3, 2013·Journal of Proteomics·Hai-Fen LiXuan-Qiang Liang
Aug 12, 2009·The Journal of Allergy and Clinical Immunology·Susanne KrauseAdriano Mari
Dec 5, 2009·Molecular Nutrition & Food Research·Andrea HarrerMartin Himly
Jun 5, 2015·PloS One·Christian Seutter von LoetzenPaul Rösch
Nov 21, 2013·The Journal of Biological Chemistry·Barry K HurlburtMaksymilian Chruszcz
Mar 29, 2020·Protein Science : a Publication of the Protein Society·Shanna Bastiaan-NetJurriaan J Mes
Sep 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Faisal ShahJinchuang Zhang
May 7, 2010·Journal of Proteome Research·Hendrik SchmidtWolf-Meinhard Becker

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