Purification and characterization of neutral and alkaline invertase from carrot

Plant Physiology
H S Lee, A Sturm

Abstract

Neutral and alkaline invertase were identified in cells of a suspension culture of carrot (Daucus carota L.) and purified to electrophoretic homogeneity. Neutral invertase is an octamer with a molecular mass of 456 kD and subunits of 57 kD, whereas alkaline invertase is a tetramer with a molecular mass of 504 kD and subunits of 126 kD. Both enzymes had sharp pH profiles, with maximal activities at pH 6.8 for neutral invertase and pH 8.0 for alkaline invertase, and both hydrolyzed sucrose with typical hyperbolic kinetics and similar Km values of about 20 mM at pH 7.5. Neutral invertase also hydrolyzed raffinose and stachyose and, therefore, is a beta-fructofuranosidase. In contrast, alkaline invertase was highly specific for sucrose. Fructose acted as a competitive inhibitor of both enzymes, with Ki values of about 15 mM. Glucose was a noncompetitive inhibitor of both neutral and alkaline invertase, with a Ki of about 30 mM. Neither enzyme was inhibited by HgCl2. Alkaline invertase was markedly inhibited by CaCl2, MgCl2, and MnCl2, and neutral invertase was not. In contrast to alkaline invertase, neutral invertase was inhibited by the nucleotides ATP, CTP, GTP, and UTP.

Citations

Jun 29, 2005·Journal of Molecular Evolution·Xuemei JiJohn Bennett
Oct 17, 2007·Planta·Philip N BocockJohn M Davis
Oct 21, 1998·Phytochemistry·D J Vorster, F C Botha
Sep 20, 2011·Journal of Agricultural and Food Chemistry·Donggiun KimTaek-Kyun Lee
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·A Sturm, S Lienhard
Mar 29, 2011·Journal of Experimental Botany·Li XiangWim Van den Ende
Sep 27, 2000·Critical Reviews in Biochemistry and Molecular Biology·H Winter, S C Huber
Jul 13, 2013·Journal of Bioscience and Bioengineering·Atsushi NagaiHiroyuki Wariishi
Dec 19, 2008·Plant Physiology and Biochemistry : PPB·Altaf HussainMuhammad Ashraf
Jun 18, 2011·Journal of the Science of Food and Agriculture·Zhen SunShu-Hua Zhu
Jul 2, 2010·Journal of Integrative Plant Biology·Donggiun KimTaek-Kyun Lee
Apr 24, 2010·The FEBS Journal·Mohammad Reza Bolouri-MoghaddamWim Van den Ende
Aug 16, 2014·Preparative Biochemistry & Biotechnology·Yonca Yuzugullu, Yonca Avcı Duman
Jun 28, 2018·Plant Molecular Biology·Sheila Juárez-ColungaAxel Tiessen
Feb 1, 2019·NPJ Systems Biology and Applications·Lisa KüstnerArnd G Heyer
Oct 24, 2021·Journal of the Science of Food and Agriculture·Sümeyye Alagöz KabakcıMehmet Özkan

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