PMID: 3768407Oct 29, 1986Paper

Purification and characterization of nuclear basic proteins of human sperm

Biochimica Et Biophysica Acta
M GusseP Chevaillier

Abstract

Highly purified nuclei were obtained from human sperm without protein loss through the use of CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), a newly available detergent. The basic protein complement of these nuclei is highly heterogeneous and comprises histones (some of which are testis-specific), protamines and proteins of intermediate basicity and molecular size. The protamines belong to two different classes of protein. Microheterogeneity observed in some of these protamines originates from slight variations in their amino acid composition as well as from post-synthetic modifications. Two of these protamines previously considered as two different proteins are in fact the same protein with different degrees of phosphorylation. All these protamines and intermediate basic proteins are characterized by high amounts of arginine and cysteine. Three of the protamines and all five intermediate basic proteins are also histidine-rich.

References

Mar 27, 1979·Biochimica Et Biophysica Acta·J Svasti, N Talupphet
Dec 1, 1978·Experimental Cell Research·N TanphaichitrP Chalermisarachai
Jun 15, 1976·Biochimica Et Biophysica Acta·P Pongsawasdi, J Svasti
Jan 1, 1975·Experimental Cell Research·T Puwaravutipanich, S Panyim
Dec 1, 1975·Developmental Biology·A R BellvéL Hanley Bowdoin
Apr 14, 1976·Biochimica Et Biophysica Acta·W S KistlerR L Heinrikson
Jan 29, 1985·Biochemistry·K C KleeneN B Hecht
Apr 1, 1986·European Journal of Biochemistry·D J McKayG H Dixon
May 1, 1985·Bioscience Reports·D J McKayG H Dixon
Nov 28, 1972·Biochimica Et Biophysica Acta·J P CoelinghA Ruhfus
Mar 15, 1983·European Journal of Biochemistry·B LaineP Sautière
Oct 1, 1984·European Journal of Biochemistry·P SautiereM Loir
Sep 1, 1983·Archives of Biochemistry and Biophysics·J Wattanaseree, J Svasti

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Citations

Dec 9, 2000·Biochemical and Biophysical Research Communications·I A ZalenskayaA O Zalensky
May 1, 1995·Chromosoma·A O ZalenskyE M Bradbury
Jan 15, 1988·Experientia·R BalhornN Tanphaichitr
Apr 1, 1990·Journal of Molecular Evolution·R Oliva, G H Dixon
Jan 1, 2005·Indian Journal of Clinical Biochemistry : IJCB·R S SharmaA Kriplani
Sep 1, 1991·Molecular Immunology·R Rousseaux-PrevostJ Rousseaux
Dec 1, 1993·Molecular Immunology·L H StankerR Balhorn
Jan 1, 1995·Micron : the International Research and Review Journal for Microscopy·J P Dadoune
May 20, 1998·Biochimie·D Wouters-TyrouP Sautière
Jul 12, 2012·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Johannes Bohacek, Isabelle M Mansuy
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y E YuM L Meistrich
Nov 26, 2009·Molecular Human Reproduction·Lars Björndahl, Ulrik Kvist
Aug 11, 1988·Nucleic Acids Research·L DomenjoudW Engel
Sep 1, 1991·European Journal of Biochemistry·A ArkhisP Chevaillier
Mar 1, 1992·European Journal of Biochemistry·M ChauvièreP Chevaillier
Oct 2, 2007·Genome Biology·Rod Balhorn
Jan 1, 1993·Molecular Reproduction and Development·I A BelokopytovaV I Vorob'ev
Apr 17, 2008·Reproductive Biomedicine Online·S RousseauxS Khochbin
Mar 14, 2009·Microscopy Research and Technique·Jean-Pierre Dadoune
Jul 9, 2009·Journal of Cellular and Molecular Medicine·Jinu LeeSuman Lee
Jul 5, 2006·Proteomics·Juan Martínez-HerediaRafael Oliva
Feb 18, 2005·Gene·Sophie RousseauxSaadi Khochbin
Feb 5, 2000·Molecular Reproduction and Development·M HazzouriB Sèle
Mar 14, 2002·Molecular Reproduction and Development·Michele CorzettRod Balhorn
Aug 15, 2000·American Journal of Industrial Medicine·B Quintanilla-VegaL D Anderson
Sep 25, 1999·Molecular Reproduction and Development·M CorzettR Balhorn

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