PMID: 16232426Oct 20, 2005

Purification and characterization of organic solvent-stable protease from organic solvent-tolerant Pseudomonas aeruginosa PST-01

Journal of Bioscience and Bioengineering
H OginoH Ishikawa

Abstract

An organic solvent-stable protease (PST-01 protease) in a culture broth of organic solvent-tolerant Pseudomonas aeruginosa PST-01 was purified by successive hydrophobic interaction chromatography using Butyl-Toyopearl gels. The purified enzyme was homogeneous as determined by SDS-polyacrylamide gel electrophoresis. PST-01 protease had a molecular mass of 38 kDa. The optimum temperature and pH for casein hydrolysis were 55 degrees C and 8.5, respectively. PST-01 protease was stable at pH 8-12 and below 50 degrees C and was determined to be a metalloprotease which was inhibited by EDTA, 1,10-phenanthroline, and phosphoramidon. PST-01 protease inhibited by EDTA was reactivated completely by the addition of zinc or cobalt ions. The stability of PST-01 protease in solutions containing water-soluble organic solvents or alcohols was higher than that in the absence of organic solvent. Furthermore, in general, PST-01 protease was more stable than commercially available proteases, namely, subtilisin Carlsberg, thermolysin, and alpha-chymotrypsin, in the presence of water-soluble organic solvents or alcohols.

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Citations

Sep 1, 2005·Applied Microbiology and Biotechnology·Dinesh Kumar, Tek Chand Bhalla
Oct 31, 2006·Extremophiles : Life Under Extreme Conditions·Hamid Reza Karbalaei-HeidariMohammad Ali Amoozegar
May 12, 2007·Journal of Industrial Microbiology & Biotechnology·Raja Noor Zaliha Raja Abd RahmanM Basri
Mar 3, 2010·Applied Biochemistry and Biotechnology·Jaya Ram SimkhadaJin Cheol Yoo
Oct 20, 2005·Journal of Bioscience and Bioengineering·N NakajimaK Ishihara
Oct 20, 2005·Journal of Bioscience and Bioengineering·H Ogino, H Ishikawa
Jan 20, 2010·Protein Engineering, Design & Selection : PEDS·H OginoNoriyuki Doukyu
May 25, 2007·Biochemical and Biophysical Research Communications·H OginoHaruo Ishikawa
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Mar 15, 2013·International Journal of Biological Macromolecules·Periasamy Anbu
Oct 23, 2014·Applied Biochemistry and Biotechnology·Shiv Shankar, Ryali Seeta Laxman
May 23, 2014·Preparative Biochemistry & Biotechnology·Arastoo Badoei-DalfardHadi Ravan
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Aug 25, 2019·Microorganisms·Richard O'CallaghanMichael Bierdeman

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