Purification and characterization of pediocin from probiotic Pediococcus pentosaceus GS4, MTCC 12683

Folia Microbiologica
Budhaditya GhoshAsit Ranjan Ghosh

Abstract

Pediococcus pentosaceus GS4 (MTCC 12683), a probiotic lactic acid bacterium (LAB), was found to produce bacteriocin in spent culture. Antibacterial and antagonistic potential of this bacteriocin against reference strains of Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 25619), and Listeria monocytogenes (ATCC 15313) was proven by double-layer and well diffusion methods wherein nisin and ampicillin were used as positive controls. Bacteriocin in supernatant was purified and analyzed by SDS-PAGE, RP-HPLC, and circular dichroism (CD). The physico-chemical properties of purified bacteriocin were characterized being treated at different temperatures (30 to 110 °C), pH (3.0 to 12.0), with different enzymes (α-amylase, pepsin, and lysozyme), and organic solvents (hexane, ethanol, methanol, and acetone) respectively. The molar mass of bacteriocin (named pediocin GS4) was determined as 9.57 kDa. The single peak appears at the retention time of 2.403 with area amounting to 25.02% with nisin as positive control in RP-HPLC. CD analysis reveals that the compound appears to have the helix ratio of 40.2% with no beta sheet. The antibacterial activity of pediocin GS4 was optimum at 50 °C and at ...Continue Reading

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Citations

Feb 18, 2021·Microbial Cell Factories·Shiman JiangLanjuan Li
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yanlong CuiXin Lü
Dec 10, 2021·Chemistry & Biodiversity·Marek BunseDietmar R Kammerer

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Datasets Mentioned

BETA
HM044322

Methods Mentioned

BETA
circular dichroism
electrophoresis

Software Mentioned

GraphPad Prism
CD
LL
CDSSTR
GraphPad
SELCON3

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