Purification and characterization of peroxidase from papaya (Carica papaya) fruit

Applied Biochemistry and Biotechnology
Veda P PandeyUpendra N Dwivedi

Abstract

Ripening of papaya fruit was found to be characterized with a decrease in peroxidase activity and its transcript. This peroxidase was purified to homogeneity through successive steps of ammonium sulfate fractionation, ion exchange and molecular exclusion chromatography. The peroxidase was purified 30.22-folds with overall recovery of 44.37% and specific activity of 68.59. Purified peroxidase was found to be a heterotrimer of ~240 kDa, containing two subunits each of 85 and one of 70 kDa. Purified enzyme exhibited pH and temperature optima of 7.0 and 40 °C, respectively. K(m) values for substrates o-dianicidin, guaiacol and ascorbic acid were found to be 0.125, 0.8 and 5.2 mM, respectively. K(m) for H(2)O(2) was found to be 0.25 mM. Salicylic acid was found to activate peroxidase up to 50 μM concentration, beyond which it acted as inhibitor. Ca(2+) and Mg(2+) activated peroxidase while sodium azide, SDS, and Triton X-100 were found to inhibit peroxidase.

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Citations

Jun 1, 2014·Applied Biochemistry and Biotechnology·Burcu SomtürkNalan Özdemir
May 31, 2015·Applied Biochemistry and Biotechnology·Umme KalsoomMuhammad Asgher
Nov 30, 2013·Journal of the Science of Food and Agriculture·José O Rojas-ReyesMaría Guadalupe Aguilar-Uscanga
Aug 2, 2018·Journal of Food Science and Technology·Chandrahas Vishwasrao, Laxmi Ananthanarayan
Oct 18, 2019·Frontiers in Plant Science·Guillaume DecrosPierre Pétriacq
May 17, 2021·Food Research International·Francine Kerstner de OliveiraJaqueline Garda Buffon

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